Histopathological assessment of wheat seedling tissues infected by Fusarium pseudograminearum

Knight, N. L. and Sutherland, M. W. (2013) Histopathological assessment of wheat seedling tissues infected by Fusarium pseudograminearum. Plant Pathology, 62 (3). pp. 679-687. ISSN 0032-0862


Abstract

Histopathological assessment of infection by the crown rot pathogen Fusarium pseudograminearum in wheat seedling tissues was performed using fluorescence microscopy. The coleoptiles and leaf sheaths of four host cultivars of differing susceptibility were examined. Leaf sheaths were most frequently penetrated via stomata, indicated by initial lesions forming at the guard cells. Internally, cell wall penetration was facilitated by penetration structures which appeared as hyphal swellings or septate foot-shaped appressoria. Colonization of leaf sheaths resulted in the re-emergence of hyphae from stomata on both surfaces of the sheath. These hyphae are hypothesized to have two major roles; first as exploratory hyphae for colonization of new tissues, and secondly as sites of profuse conidial production. The formation of conidia on the leaf sheath surface was only recorded on the most susceptible bread wheat genotype. No other major differences in host-pathogen interactions were observed among these cultivars. Almost all cell types in the leaf sheath tissues were extensively colonized, except for the vascular bundles and silica cells. This investigation provides the first comprehensive assessment of F. pseudograminearum infection structures and growth patterns during the infection of wheat seedlings.


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Item Type: Article (Commonwealth Reporting Category C)
Refereed: Yes
Item Status: Live Archive
Additional Information: © 2012 The Authors. Plant Pathology © 2012 BSPP Permanent restricted access to published version due to publisher copyright policy. Article first published online: 2 Aug 2012
Faculty/School / Institute/Centre: Historic - Faculty of Sciences - Department of Biological and Physical Sciences (Up to 30 Jun 2013)
Faculty/School / Institute/Centre: Historic - Faculty of Sciences - Department of Biological and Physical Sciences (Up to 30 Jun 2013)
Date Deposited: 14 May 2013 22:38
Last Modified: 11 Jul 2014 05:09
Uncontrolled Keywords: crown rot; fluorescence microscopy; Fusarium; staining
Fields of Research (2008): 06 Biological Sciences > 0607 Plant Biology > 060702 Plant Cell and Molecular Biology
07 Agricultural and Veterinary Sciences > 0703 Crop and Pasture Production > 070305 Crop and Pasture Improvement (Selection and Breeding)
07 Agricultural and Veterinary Sciences > 0703 Crop and Pasture Production > 070308 Crop and Pasture Protection (Pests, Diseases and Weeds)
Fields of Research (2020): 31 BIOLOGICAL SCIENCES > 3108 Plant biology > 310803 Plant cell and molecular biology
30 AGRICULTURAL, VETERINARY AND FOOD SCIENCES > 3004 Crop and pasture production > 300406 Crop and pasture improvement (incl. selection and breeding)
30 AGRICULTURAL, VETERINARY AND FOOD SCIENCES > 3004 Crop and pasture production > 300409 Crop and pasture protection (incl. pests, diseases and weeds)
Socio-Economic Objectives (2008): B Economic Development > 82 Plant Production and Plant Primary Products > 8205 Winter Grains and Oilseeds > 820507 Wheat
Identification Number or DOI: https://doi.org/10.1111/j.1365-3059.2012.02663.x
URI: http://eprints.usq.edu.au/id/eprint/23486

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