Correlation between NIRS generated and chemically measured feed quality data in barley (Hordeum vulgare), and potential use in QTL analysis identification

Gous, Peter W. and Martin, Anke and Lawson, Wendy and Kelly, Alison and Fox, Glen P. and Sutherland, Mark W. (2012) Correlation between NIRS generated and chemically measured feed quality data in barley (Hordeum vulgare), and potential use in QTL analysis identification. Euphytica: International Journal on Plant Breeding, 188 (3). pp. 325-332. ISSN 0014-2336

Abstract

A study was performed to investigate the value of near infrared reflectance spectroscopy (NIRS) as an alternate method to analytical techniques for identifying QTL associated with feed quality traits.
Milled samples from an F6—derived recombinant inbred Tallon/Scarlett population were incubated inthe rumen of fistulated cattle, recovered, washed and dried to determine the in-situ dry matter digestibility(DMD). Both pre- and post-digestion samples were analysed using NIRS to quantify key quality components relating to acid detergent fibre, starch and protein. This phenotypic data was used to identify trait associated QTL and compare them to previously
identified QTL. Though a number of genetic correlations were identified between the phenotypic data sets, the only correlation of most interest was between DMD and starch digested (r = -0.382). The significance of this genetic correlation was that the NIRS data set identified a putative QTL on chromosomes 7H (LOD = 3.3) associated with starch digested. A QTL for DMD occurred in the same region of chromosome 7H, with flanking markers fAG/CAT63 and bPb-0758.
The significant correlation and identification of this putative QTL, highlights the potential of technologies like NIRS in QTL analysis.


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Item Type: Article (Commonwealth Reporting Category C)
Refereed: Yes
Item Status: Live Archive
Additional Information: © 2012 Springer Science+Business Media B.V. Permanent restricted access to published version in accordance with the copyright policy of the publisher.
Faculty / Department / School: Current - USQ Other
Date Deposited: 31 Mar 2013 08:19
Last Modified: 17 Nov 2014 03:09
Uncontrolled Keywords: near infrared reflectance spectroscopy; QTL; barley; starch; ADF; protein; pre-digestion; post-digestion
Fields of Research : 06 Biological Sciences > 0601 Biochemistry and Cell Biology > 060199 Biochemistry and Cell Biology not elsewhere classified
06 Biological Sciences > 0604 Genetics > 060407 Genome Structure and Regulation
07 Agricultural and Veterinary Sciences > 0702 Animal Production > 070204 Animal Nutrition
Socio-Economic Objective: E Expanding Knowledge > 97 Expanding Knowledge > 970106 Expanding Knowledge in the Biological Sciences
Identification Number or DOI: 10.1007/s10681-012-0644-x
URI: http://eprints.usq.edu.au/id/eprint/23207

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