Evaluation of the immunogenicity of the P97R1 adhesin of Mycoplasma hyopnemoniae as a mucosal vaccine in mice

Chen, Austen Y. and Fry, Scott R. and Forbes-Faulkner, Judy and Daggard, Grant ORCID: https://orcid.org/0000-0002-8005-4273 and Mukkur, T. K. S. (2006) Evaluation of the immunogenicity of the P97R1 adhesin of Mycoplasma hyopnemoniae as a mucosal vaccine in mice. Journal of Medical Microbiology, 55 (7). pp. 923-929. ISSN 0022-2615


Abstract

The immunogenicity of P97 adhesin repeat region R1 (P97R1) of Mycoplasma hyopneumoniae, an important pathogenesis-associated region of P97, was evaluated in mice as a mucosal vaccine. Mice were immunized orally with attenuated Salmonella typhimurium aroA strain CS332 harbouring a eukaryotic or prokaryotic expression vector encoding P97R1. Local and systemic immune responses were analysed by ELISA on mouse sera, lung washes and splenocyte supernatants following splenocyte stimulation with specific antigens in vitro. Although no P97R1-specific antibody responses were detected in serum and lung washes, significant gamma interferon was produced by P97R1-stimulated splenocytes from mice immunized orally with S. typhimurium aroA harbouring either expression system, indicating induction of a cell-mediated immune response. These results suggested that live bacterial vectors carrying DNA vaccines or expressing heterologous antigens preferentially induce a Th1 response. Surprisingly, however, mice immunized with the vaccine carrier S. typhimurium aroA CS332 induced serum IgG, but not mucosal IgA, against P97R1 or S. typhimurium aroA CS332 whole-cell lysate, emphasizing the importance of assessing the suitability of attenuated S. typhimurium antigen-carrier delivery vectors in the mouse model prior to their evaluation as potential vaccines in the target species, which in this instance was pigs.


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Item Type: Article (Commonwealth Reporting Category C)
Refereed: Yes
Item Status: Live Archive
Additional Information: Files associated with this item cannot be displayed due to copyright restrictions.
Faculty/School / Institute/Centre: Historic - Faculty of Sciences - Department of Biological and Physical Sciences (Up to 30 Jun 2013)
Faculty/School / Institute/Centre: Historic - Faculty of Sciences - Department of Biological and Physical Sciences (Up to 30 Jun 2013)
Date Deposited: 11 Oct 2007 01:02
Last Modified: 11 Aug 2021 05:03
Uncontrolled Keywords: AP; alkaline phosphatase; IFN-{gamma}; gamma interferon; P97R1; P97 repeat region R1; PEP; porcine enzootic pneumonia
Fields of Research (2008): 07 Agricultural and Veterinary Sciences > 0707 Veterinary Sciences > 070705 Veterinary Immunology
06 Biological Sciences > 0605 Microbiology > 060599 Microbiology not elsewhere classified
Fields of Research (2020): 30 AGRICULTURAL, VETERINARY AND FOOD SCIENCES > 3009 Veterinary sciences > 300906 Veterinary immunology
31 BIOLOGICAL SCIENCES > 3107 Microbiology > 310799 Microbiology not elsewhere classified
Identification Number or DOI: https://doi.org/10.1099/jmm.0.46088-0
URI: http://eprints.usq.edu.au/id/eprint/2296

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