Diversity arrays technology (DArT) for high-throughput profiling of the hexaploid wheat genome

Akbari, Mona and Wenzl, Peter and Caig, Vanessa and Carling, Jason and Xia, Ling and Yang, Shiying and Uszynski, Grzegorz and Mohler, Volker and Lehmensiek, Anke and Kuchel, Haydn and Hayden, Mathew J. and Howes, Neil and Sharp, Peter and Vaughan, Peter and Rathmell, Bill and Huttner, Eric and Kilian, Andrzej (2006) Diversity arrays technology (DArT) for high-throughput profiling of the hexaploid wheat genome. Theoretical and Applied Genetics: International Journal of Plant Breeding Research, 113 (8). pp. 1409-1420. ISSN 0040-5752


Despite a substantial investment in the development of panels of single nucleotide polymorphism (SNP) markers, the simple sequence repeat (SSR) technology with a limited multiplexing capability remains a standard, even for applications requiring whole-genome information. Diversity arrays technology (DArT) types hundreds to thousands of genomic loci in parallel, as previously demonstrated in a number diploid plant species. Here we show that DArT
performs similarly well for the hexaploid genome of bread wheat (Triticum aestivum L.). The methodology previously used to generate DArT fingerprints of barley also generated a large number of high-quality markers in wheat (99.8% allele-calling concordance and approximately 95% call rate). The genetic relationships among bread wheat cultivars revealed by DArT coincided with knowledge generated with other methods, and even closely related cultivars could be distinguished. To verify the Mendelian
behaviour of DArT markers, we typed a set of 90 Cranbrook £ Halberd doubled haploid lines for which a framework (FW) map comprising a total of 339 SSR, restriction fragment length polymorphism (RFLP) and amplified fragment length polymorphism (AFLP) markers was available. We added an equal number of DArT markers to this data set and also incorporated 71 sequence tagged microsatellite (STM) markers. A comparison of logarithm of the odds (LOD) scores,
call rates and the degree of genome coverage indicated that the quality and information content of the DArT data set was comparable to that of the combined SSR/RFLP/AFLP data set of the FW map.

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Item Type: Article (Commonwealth Reporting Category C)
Refereed: Yes
Item Status: Live Archive
Additional Information: Permanent restricted access to published version due to publisher copyright policy.
Faculty / Department / School: Historic - Faculty of Sciences - Department of Biological and Physical Sciences
Date Deposited: 11 Oct 2007 01:01
Last Modified: 04 Dec 2013 02:52
Uncontrolled Keywords: dna; array technology; DArT; hexaploid; wheat; genome
Fields of Research : 10 Technology > 1001 Agricultural Biotechnology > 100105 Genetically Modified Field Crops and Pasture
07 Agricultural and Veterinary Sciences > 0703 Crop and Pasture Production > 070305 Crop and Pasture Improvement (Selection and Breeding)
06 Biological Sciences > 0604 Genetics > 060405 Gene Expression (incl. Microarray and other genome-wide approaches)
Socio-Economic Objective: B Economic Development > 82 Plant Production and Plant Primary Products > 8205 Winter Grains and Oilseeds > 820507 Wheat
Identification Number or DOI: 10.1007/s00122-006-0365-4
URI: http://eprints.usq.edu.au/id/eprint/2292

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