Impact assessment of pumping parameters on groundwater bacterial communities

Shani, Noam and Aravinthan, Vasantha and Rossi, Pierre and Laulan, Guillaume and Ducommun, Pascale and Holliger, Christof (2013) Impact assessment of pumping parameters on groundwater bacterial communities. Ground Water Monitoring and Remediation, 33 (2). pp. 31-37. ISSN 1069-3629


Groundwater microbial community samples are traditionally collected using pumping techniques optimized for groundwater chemistry assessment, although the impact of groundwater pumping parameters on apparent bacterial community structures (BCSs) is not really known. We therefore studied the impact of pumping lift, flow regime, and tubing material on BCS, which were analyzed by terminal-restriction fragment length polymorphism (T-RFLP). Ruzicka dissimilarity coefficients were calculated between T-RFLP profiles to assess disparities between BCS. Variations in pumping lift, flow regime, and tubing material did not affect the apparent BCS in experiments using a homogenous water system under laboratory conditions showing that the conditions within the tube had no detectable effect on BCS. However, pumping groundwater from aquifer monitoring wells at different flow rates in the field revealed a significant impact on the apparent BCS. Water samples collected from fine sediment were the most affected by the pumping flow rate.

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Item Type: Article (Commonwealth Reporting Category C)
Refereed: Yes
Item Status: Live Archive
Additional Information: © 2012, The Author(s) © 2012, National Ground Water Association. Published online 17 Sep 2012. Permanent restricted access to published version in accordance with the copyright policy of the publisher.
Faculty/School / Institute/Centre: Historic - Faculty of Business and Law - No Department
Date Deposited: 27 Sep 2012 01:55
Last Modified: 15 Oct 2014 01:21
Uncontrolled Keywords: wells; pumps; aquifers; ecology
Fields of Research : 04 Earth Sciences > 0403 Geology > 040301 Basin Analysis
04 Earth Sciences > 0406 Physical Geography and Environmental Geoscience > 040603 Hydrogeology
06 Biological Sciences > 0605 Microbiology > 060504 Microbial Ecology
Socio-Economic Objective: D Environment > 96 Environment > 9605 Ecosystem Assessment and Management > 960506 Ecosystem Assessment and Management of Fresh, Ground and Surface Water Environments
Identification Number or DOI: 10.1111/j.1745-6592.2012.01420.x

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