Arsenic mineral dissolution and possible mobilization in mineral-microbe-groundwater environment

Islam, A. B. M. R. and Maity, Jyoti Prakash and Bundschuh, Jochen and Chen, Chien-Yen and Bhowmik, Bejon Kumar and Tazaki, Kazue (2013) Arsenic mineral dissolution and possible mobilization in mineral-microbe-groundwater environment. Journal of Hazardous Materials, 262. pp. 989-996. ISSN 0304-3894


Arsenic (As) is widely distributed in the nature as ores or minerals. It has been attracted much attention for the global public health issue, especially for groundwater As contamination. The aim of this study was to elucidate the characteristics of microbes in groundwater where As-minerals were dissolved. An ex situ experiment was conducted with 7 standard As-minerals in bacteria-free groundwater and stored in experimental vessels for 1 year without supplementary nutrients. The pH (6.7-8.4) and Eh S.H.E. (24-548 mV) changed between initial (0 day) and final stages (365 days) of experiment. The dissolution of As was detected higher from arsenolite (4240 ± 8.69 mg/L) and native arsenic (4538 ± 9.02 mg/L), whereas moderately dissolved from orpiment (653 ± 3.56 mg/L) and realgar (319 ± 2.56 mg/L) in compare to arsenopyrite (85 ± 1.25 mg/L) and tennantite (3 ± 0.06 mg/L). Optical microscopic, scanning electron microscopic observations and flurometric enumeration revealed the abundance of As-resistant bacillus, coccus and filamentous types of microorganisms on the surface of most of As-mineral. 4′-6-Diamidino-2-phenylindole (DAPI)-stained epifluorescence micrograph confirmed the presence of DNA and carboxyfluorescein diacetate (CFDA) staining method revealed the enzymatically active bacteria on the surface of As-minerals such as in realgar (As 4S 4). Therefore, the microbes enable to survive and mobilize the As in groundwater by dissolution/bioweathering of As-minerals.

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Item Type: Article (Commonwealth Reporting Category C)
Refereed: Yes
Item Status: Live Archive
Additional Information: © 2012 Published by Elsevier B.V. Published online 14 July 2012. Published version deposited in accordance with the copyright policy of the publisher.
Faculty / Department / School: Current - Faculty of Health, Engineering and Sciences - School of Agricultural, Computational and Environmental Sciences
Date Deposited: 12 Sep 2012 05:08
Last Modified: 14 Oct 2014 23:09
Uncontrolled Keywords: arsenic-minerals; As-resistant microorganisms; groundwater; bioweathering; flurometric enumeration
Fields of Research : 11 Medical and Health Sciences > 1117 Public Health and Health Services > 111705 Environmental and Occupational Health and Safety
11 Medical and Health Sciences > 1117 Public Health and Health Services > 111799 Public Health and Health Services not elsewhere classified
09 Engineering > 0905 Civil Engineering > 090509 Water Resources Engineering
Socio-Economic Objective: D Environment > 96 Environment > 9611 Physical and Chemical Conditions of Water > 961199 Physical and Chemical Conditions of Water not elsewhere classified
Identification Number or DOI: 10.1016/j.jhazmat.2012.07.022

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