Quantitative PCR and histopathological investigations of cereal tissues during infection by the crown rot pathogen Fusarium pseudograminearum

Knight, N. L. and Lehmensiek, A. and Herde, D. J. and Sutherland, M. W. (2011) Quantitative PCR and histopathological investigations of cereal tissues during infection by the crown rot pathogen Fusarium pseudograminearum. In: 4th Asian Conference on Plant Pathology and the 18th Biennial Australasian Plant Pathology Society Conference (ACPP/APPS 2011): New Frontiers in Plant Pathology for Asia and Oceania, 26-29 Apr 2011, Darwin, Australia.

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Crown rot of wheat is a significant cause of yield losses in
many wheat producing countries, particularly Australia
where the predominant cause is the fungus Fusarium
pseudograminearum. Other cereals such as durum wheat and barley can also be affected. Partial resistance has been
identified in a small number of wheat lines, such as 2-49
and Sunco, but the mechanisms of resistance shown by these lines have not been identified. Using quantitative PCR
based on fungal translation elongation factor α DNA we
have established that fungal biomass in partially resistant
genotypes is reduced compared to susceptible genotypes in
both seedling and adult cereal tissues. Histopathological
examination of infection and colonisation of seedling and
adult tissues, using the fluorescent dye solophenyl flavine,
has not revealed any differences in tissue responses
between partially resistant and susceptible host tissues,
although there is a significantly slower spread of the fungus in the tissues of resistant genotypes. Infection is initiated predominantly through the stomata of surface-inoculated leaf sheaths. Colonisation of expanded stems frequently originates in the parenchymatous hypoderm, which
becomes highly discoloured. Early infection of pith
parenchyma cells is also frequent. Vascular tissues become
colonised by anthesis and this occurs more rapidly in
susceptible genotypes. Occlusion of large xylem vessels was
rare during moderate infections while infection of phloem
sieve tube elements is common.

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Item Type: Conference or Workshop Item (Commonwealth Reporting Category E) (Poster)
Refereed: Yes
Item Status: Live Archive
Additional Information: Not reportable (poster paper).
Faculty/School / Institute/Centre: Historic - Faculty of Sciences - Department of Biological and Physical Sciences
Date Deposited: 01 Jul 2012 12:43
Last Modified: 06 Jul 2017 00:40
Uncontrolled Keywords: fungal diseases; wheat; production losses; cereals; resistance
Fields of Research : 06 Biological Sciences > 0605 Microbiology > 060505 Mycology
07 Agricultural and Veterinary Sciences > 0703 Crop and Pasture Production > 070308 Crop and Pasture Protection (Pests, Diseases and Weeds)
06 Biological Sciences > 0607 Plant Biology > 060703 Plant Developmental and Reproductive Biology
Socio-Economic Objective: B Economic Development > 82 Plant Production and Plant Primary Products > 8205 Winter Grains and Oilseeds > 820507 Wheat
URI: http://eprints.usq.edu.au/id/eprint/21185

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