Parkinson, John and Samiric, Tom and Ilic, Mirna Z. and Cook, Jill and Feller, Julian A. and Handley, Christopher J. (2010) Change in proteoglycan metabolism is a characteristic of human patellar tendinopathy. Arthritis and Rheumatism, 62 (10). pp. 3028-3035. ISSN 0004-3591
Objective. To determine differences in the metabolism of proteoglycans and the gene expression of proteinases and their inhibitors between patellar tendons exhibiting chronic overuse tendinopathy and normal patellar tendons in humans.
Methods. Rates of loss and synthesis of proteoglycans were determined. Radiolabeled and total proteoglycans retained in and lost from the tissue were analyzed by fluorography and Western blotting. Levels of messenger RNA for matrix metalloproteinase 1(MMP-1), MMP-2, MMP-3, MMP-9, MMP-13,
ADAMTS-1, ADAMTS-4, ADAMTS-5, tissue inhibitor of metalloproteinases 1 (TIMP-1), TIMP-2, TIMP-3, and TIMP-4 were determined in fresh tissue.
Results. The rate of loss of 35S-labeled proteoglycans was greater in abnormal tendons, as was the rate of synthesis of proteoglycans. Fluorography and Western blotting revealed the presence of greater amounts of large proteoglycans (aggrecan and versican) in abnormal tendons, and these proteoglycans were rapidly lost from the matrix of abnormal tendons. There was no significant difference in the expression of ADAMTS-1, ADAMTS-4, ADAMTS-5, MMP-1, MMP-2, MMP-3, MMP-13, TIMP-2, TIMP-3, or TIMP-4. There was a
significant increase in the expression of MMP-9 and TIMP-1 in abnormal tendons.
Conclusion. Our findings suggest that a change in the proteoglycan content of the extracellular matrix in abnormal tendons results from the altered metabolism of the cells, reflected in the enhanced synthesis of the large proteoglycans aggrecan and versican, and does not appear to result from changes at the level of gene expression.
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|Item Type:||Article (Commonwealth Reporting Category C)|
|Item Status:||Live Archive|
|Additional Information:||Permanent restricted access to published version due to publisher copyright policy.|
|Faculty / Department / School:||Historic - Faculty of Sciences - Department of Biological and Physical Sciences|
|Date Deposited:||24 Jul 2011 05:54|
|Last Modified:||20 Oct 2014 04:31|
|Uncontrolled Keywords:||gene expression profiling; human tissue; nucleotide sequence; patella tendon; protein degradation; protein expression; protein metabolism; protein synthesis; repetitive strain injury; tendinitis|
|Fields of Research :||06 Biological Sciences > 0601 Biochemistry and Cell Biology > 060104 Cell Metabolism
11 Medical and Health Sciences > 1103 Clinical Sciences > 110322 Rheumatology and Arthritis
10 Technology > 1004 Medical Biotechnology > 100401 Gene and Molecular Therapy
|Socio-Economic Objective:||C Society > 92 Health > 9201 Clinical Health (Organs, Diseases and Abnormal Conditions) > 920116 Skeletal System and Disorders (incl. Arthritis)|
|Identification Number or DOI:||doi: 10.1002/art.27587|
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