Shared primer PCR combined with hybridisation for the detection of Bordetella Pertussis and B. Parapertussis

Farrell, David John; Daggard, Grant Edward; Mukkur, Trilochan Kanwaljit Singh (2001) Shared primer PCR combined with hybridisation for the detection of Bordetella Pertussis and B. Parapertussis. .

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Abstract

A method of detecting Bordetella pertussis and Bordetella parapertussis infection is provided. The method includes amplification of a Bordetella porin gene fragment from a sample such as a human nasopharyngeal aspirate using a Bordetella-specific primer, a Bordetella pertussis-specific primer and a Bordetella parapertussis-specific primer. The presence of the Bordetella gene fragment is then detected by hybridization to a Bordetella-specific probe. If positive, hybridization to a Bordetella pertussis-specific probe and/or a Bordetella parapertussis-specific probe is used to determine whether the porin gene fragment is indicative of Bordetella pertussis and/or Bordetella parapertussis infection. The method of the invention is designed so that errors introduced by amplification are minimized by generating amplification products of similar size and by incorporating amplification of a reference nucleic acid as an internal control.


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Item Type: Patent
Item Status: Live Archive
Additional Information: This publication is copyright. It may be reproduced in whole or in part for the purposes of study, research, or review, but is subject to the inclusion of an acknowledgment of the source. More information at: http://patentscope.wipo.int/search/en/detail.jsf?docId=WO2001090405&recNum=6&maxRec=6&office=&prevFilter=&sortOption=Pub+Date+Desc&queryString=FP%3A%28Shared+primer+PCR%29&tab=PCT+Biblio
Faculty / Department / School: Historic - Faculty of Sciences - Department of Biological and Physical Sciences
Date Deposited: 30 Nov 2007 16:19
Last Modified: 12 Dec 2014 03:57
Uncontrolled Keywords: whooping cough; diagnosis
Fields of Research : 10 Technology > 1004 Medical Biotechnology > 100402 Medical Biotechnology Diagnostics (incl. Biosensors)
11 Medical and Health Sciences > 1102 Cardiovascular Medicine and Haematology > 110203 Respiratory Diseases
11 Medical and Health Sciences > 1103 Clinical Sciences > 110309 Infectious Diseases
Socio-Economic Objective: C Society > 92 Health > 9201 Clinical Health (Organs, Diseases and Abnormal Conditions) > 920115 Respiratory System and Diseases (incl. Asthma)
URI: http://eprints.usq.edu.au/id/eprint/18120

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