Photosensitization of the sunscreen octyl-p-dimethylaminobenzoate by UVA in human melanocytes but not in keratinocytes

Xu, Chunxia and Green, Adele and Parisi, Alfio and Parsons, Peter G. (2001) Photosensitization of the sunscreen octyl-p-dimethylaminobenzoate by UVA in human melanocytes but not in keratinocytes. Photochemistry and Photobiology, 73 (6). pp. 600-604. ISSN 0031-8655


Sunscreens penetrate human epidermis and modify the biology of proliferating cells. This study addressed the question whether the UV response of cultured human cells is affected by direct treatment with nontoxic levels of sunscreens. Cell survival following exposure to UVC or unfiltered UVB was not altered by preincubation with 25 mg/mL of octyl p-dimethylaminobenzoate (o-PABA), 2-ethylhexyl p-methoxycinnamate (EHMC) or oxybenzone. However, UVA or UVB filtered to reproduce the solar UV spectrum penetrating to the basal layer of the epidermis, highly sensitized cells to killing by o-PABA but not by its hydrolysis product, 4-dimethylaminobenzoic acid. Sensitization was found in all cell types tested, except normal keratinocytes, and could be prevented by certain antioxidants particularly pyruvate and the hydroxyl radical scavenger mannitol. o-PABA and EHMC
applied without UV reduced the adherence of cells. The
results indicate that sunscreens may increase cell mobility
and the combination of o-PABA with solar UV may selectively damage melanocytes in the skin.

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Item Type: Article (Commonwealth Reporting Category C)
Refereed: Yes
Item Status: Live Archive
Additional Information: Published version deposited in accordance with the copyright policy of the publisher.
Faculty / Department / School: Historic - Faculty of Sciences - Department of Biological and Physical Sciences
Date Deposited: 30 Nov 2007 11:47
Last Modified: 12 Jun 2014 06:05
Uncontrolled Keywords: sunscreen; skin; skin cancer; cell biology; UVA: UVC; UVB; antioxidant; prevention
Fields of Research : 11 Medical and Health Sciences > 1116 Medical Physiology > 111601 Cell Physiology
06 Biological Sciences > 0601 Biochemistry and Cell Biology > 060106 Cellular Interactions (incl. Adhesion, Matrix, Cell Wall)
04 Earth Sciences > 0401 Atmospheric Sciences > 040103 Atmospheric Radiation
Socio-Economic Objective: C Society > 92 Health > 9204 Public Health (excl. Specific Population Health) > 920407 Health Protection and/or Disaster Response
Identification Number or DOI: 10.1562/0031-8655(2001)0730600POTSOP2.0.CO2

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