Assessment of ultraviolet radiation exposures in photobiological experiments

Wong, J. C. F. and Parisi, A. V. (1999) Assessment of ultraviolet radiation exposures in photobiological experiments. In: 2nd Internet Conference on Photochemistry and Photobiology: Protection Against the Hazards of UVR 1999, 18 Jan-5 Feb 1999.

Text (Published Version)

Download (133kB)


The interfering effect of ultraviolet (UV) radiation on the natural function of biological processes is wavelength specific and the UV spectrum must be weighted with the action spectrum for the process. The UV spectral irradiance may be measured with calibrated spectroradiometers. Alternatively, the biologically effective UV may be measured with broadband devices. This paper reviews the techniques for assessing biologically effective exposures in photobiological experiments.

UV meters, such as the Robertson-Berger (RB) meter, or passive dosimeters, such as polysulphone, that possess a spectral response approximating the human erythemal response can be used to estimate erythemally effective exposure or actinic exposure due to solar UV. The sensitivity of the RB meter is about 0.56 uW cm-2 and polysulphone can record an exposure of about 2mJ cm-2. For photobiological processes other than erythema these devices are not suitable to determine the exposure. In terms of these applications, a spectrum evaluator consisting of four different types of dosimeter material can be employed to evaluate the UV spectrum of the source. This method can be useful both for solar UV studies and research with UV lamps that possess radiation wavelengths shorter than 295nm. The device can be used to measure exposures where the actinic and erythemal action spectra differ significantly. It can also be used to assess exposure due to low levels of UV (about 0.01uW cm-2) caused by radiation filtered through glasses or plastic.

Statistics for USQ ePrint 1279
Statistics for this ePrint Item
Item Type: Conference or Workshop Item (Commonwealth Reporting Category E) (Paper)
Refereed: Yes
Item Status: Live Archive
Additional Information: No evidence of copyright restrictions. Authors are conference organisers.
Faculty/School / Institute/Centre: Historic - Faculty of Sciences - Department of Biological and Physical Sciences (Up to 30 Jun 2013)
Faculty/School / Institute/Centre: Historic - Faculty of Sciences - Department of Biological and Physical Sciences (Up to 30 Jun 2013)
Date Deposited: 11 Oct 2007 00:38
Last Modified: 14 Nov 2013 00:16
Uncontrolled Keywords: UV; dosimeters; photobiology; action spectrum; spectroradiometer; skin cancer; radiometer; erythema
Fields of Research (2008): 11 Medical and Health Sciences > 1116 Medical Physiology > 111601 Cell Physiology
04 Earth Sciences > 0401 Atmospheric Sciences > 040103 Atmospheric Radiation
02 Physical Sciences > 0299 Other Physical Sciences > 029904 Synchrotrons; Accelerators; Instruments and Techniques
Fields of Research (2020): 32 BIOMEDICAL AND CLINICAL SCIENCES > 3208 Medical physiology > 320801 Cell physiology
37 EARTH SCIENCES > 3701 Atmospheric sciences > 370106 Atmospheric radiation
51 PHYSICAL SCIENCES > 5110 Synchrotrons and accelerators > 511099 Synchrotrons and accelerators not elsewhere classified
Socio-Economic Objectives (2008): C Society > 92 Health > 9201 Clinical Health (Organs, Diseases and Abnormal Conditions) > 920117 Skin and Related Disorders

Actions (login required)

View Item Archive Repository Staff Only