Mapping spot blotch resistance genes in four barley populations

Bovill, Jessica and Lehmensiek, Anke and Sutherland, Mark W. and Platz, Greg. J. and Usher, Terry and Franckowiak, Jerome and Mace, Emma (2010) Mapping spot blotch resistance genes in four barley populations. Molecular Breeding: New Strategies in Plant Improvement, 26 (4). pp. 653-666. ISSN 1380-3743

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Abstract

Bipolaris sorokiniana (teleomorph: Cochliobolus sativus) is the fungal pathogen responsible for spot blotch in barley (Hordeum vulgare L.) and occurs worldwide in warmer, humid growing conditions. Current Australian barley varieties are largely susceptible to this disease and attempts are being made to introduce sources of resistance from North America. In this study we have compared chromosomal locations of spot blotch resistance reactions in four North American two-rowed barley lines; the North Dakota lines ND11231-12 and ND11231-11 and the Canadian lines TR251 and WPG8412-9-2-1. Diversity Arrays Technology (DArT)-based PCR, expressed sequence tag (EST) and SSR markers have been mapped across four populations derived from crosses between susceptible parental lines and these four resistant parents to determine the location of resistance loci. Quantitative trait loci (QTL) conferring resistance to spot blotch in adult plants (APR) were detected on chromosomes 3HS and 7HS. In contrast, seedling resistance (SLR) was controlled solely by a locus on chromosome 7HS. The phenotypic variance explained by the APR QTL on 3HS was between 16 and 25% and the phenotypic variance explained by the 7HS APR QTL was between 8 and 42% across the four populations. The SLR QTL on 7HS explained between 52 to 64% of the phenotypic variance. An examination of the pedigrees of these resistance sources supports the common identity of resistance in these lines and indicates that only a limited number of major resistance loci are available in current two-rowed germplasm.


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Item Type: Article (Commonwealth Reporting Category C)
Refereed: Yes
Item Status: Live Archive
Additional Information: Author's version deposited in accordance with the copyright policy of the publisher (Springer)
Depositing User: Prof Mark Sutherland
Faculty / Department / School: Historic - Faculty of Sciences - Department of Biological and Physical Sciences
Date Deposited: 24 Jan 2011 21:07
Last Modified: 24 Jul 2014 13:51
Uncontrolled Keywords: spot blotch resistance; barley; Bipolaris sorokiniana; Cochliobolus sativus; disease resistance; MAS; QTL
Fields of Research (FOR2008): 06 Biological Sciences > 0607 Plant Biology > 060704 Plant Pathology
06 Biological Sciences > 0605 Microbiology > 060505 Mycology
07 Agricultural and Veterinary Sciences > 0703 Crop and Pasture Production > 070305 Crop and Pasture Improvement (Selection and Breeding)
Socio-Economic Objective (SEO2008): B Economic Development > 82 Plant Production and Plant Primary Products > 8205 Winter Grains and Oilseeds > 820501 Barley
Identification Number or DOI: doi: 10.1007/s11032-010-9401-9
URI: http://eprints.usq.edu.au/id/eprint/8864

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