Comparative immunogenicity of M.hypopneumoniae NrdF encoded in different expression systems delivered orally via attenuated S.typhimurium aroA in mice

Chen, Austen Y. and Fry, Scott Robert and Forbes-Faulkner, Judy and Daggard, Grant and Mukkur, T. K. S. (2006) Comparative immunogenicity of M.hypopneumoniae NrdF encoded in different expression systems delivered orally via attenuated S.typhimurium aroA in mice. Veterinary Microbiology, 114 (3-4). pp. 252-259. ISSN 0378-1135

Abstract

The Mycoplasma hyopneumoniae ribonucleotide reductase R2 subunit (NrdF) gene fragment was cloned into eukaryotic and prokaryotic expression vectors and its immunogenicity evaluated in mice immunized orally with attenuated Salmonella typhimurium aroA CS332 harboring either of the recombinant expression plasmids. We found that NrdF is highly conserved among M. hyopneumoniae strains. The immunogenicity of NrdF was examined by analyzing antibody responses in sera and lung washes, and the cell-mediated immune (CMI) response was assessed by determining the INF-γ level produced by splenocytes upon in vitro stimulation with NrdF antigen. S. typhimurium expressing NrdF encoded by the prokaryotic expression plasmid (pTrcNrdF) failed to elicit an NrdF-specific serum or secretory antibody response, and IFN-γ was not produced. Similarly, S. typhimurium carrying the eukaryotic recombinant plasmid encoding NrdF (pcNrdF) did not induce a serum or secretory antibody response, but did elicit significant NrdF-specific IFN-γ production, indicating induction of a CMI response. However, analysis of immune responses against the live vector S. typhimurium aroA CS332 showed a serum IgG response but no mucosal IgA response in spite of its efficient invasiveness in vitro. In the present study we show that the DNA vaccine encoding the M. hyopneumoniae antigen delivered orally via a live attenuated S. typhimurium aroA can induce a cell-mediated immune response. We also indicate that different live bacterial vaccine carriers may have an influence on the type of the immune response induced.


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Item Type: Article (Commonwealth Reporting Category C)
Refereed: Yes
Item Status: Live Archive
Additional Information: Author version not held.
Depositing User: Ms Debbie White
Faculty / Department / School: Historic - Faculty of Sciences - Department of Biological and Physical Sciences
Date Deposited: 10 May 2010 11:52
Last Modified: 02 Jul 2013 23:51
Uncontrolled Keywords: immunogenicity; mycoplasma hyopneumoniae; NrdF antigen; oral immunization
Fields of Research (FOR2008): 11 Medical and Health Sciences > 1107 Immunology > 110702 Applied Immunology (incl. Antibody Engineering, Xenotransplantation and T-cell Therapies)
11 Medical and Health Sciences > 1108 Medical Microbiology > 110801 Medical Bacteriology
10 Technology > 1004 Medical Biotechnology > 100403 Medical Molecular Engineering of Nucleic Acids and Proteins
Socio-Economic Objective (SEO2008): E Expanding Knowledge > 97 Expanding Knowledge > 970106 Expanding Knowledge in the Biological Sciences
E Expanding Knowledge > 97 Expanding Knowledge > 970111 Expanding Knowledge in the Medical and Health Sciences
Identification Number or DOI: doi: 10.1016/j.vetmic.2005.12.009
URI: http://eprints.usq.edu.au/id/eprint/7829

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