Muhlhausler, B. S. and Roberts, C. T. and Yuen, B. S. J. and Marrocco, E. and Budge, H. and Symonds, M. E. and McFarlane, J. R. and Kauter, K. G. and Stagg, P. and Pearse, J. K. and McMillen, I. C. (2003) Determinants of fetal leptin synthesis, fat mass, and circulating leptin concentrations in well-nourished ewes in late pregnancy. Endocrinology, 144 (11). pp. 4947-4954. ISSN 0013-7227
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Official URL: http://endo.endojournals.org/content/144/11/4947.long
Identification Number or DOI: doi: 10.1210/en.2003-0555
We have investigated the factors regulating leptin synthesis, fat deposition, and circulating leptin concentrations in fetuses of well nourished ewes in late pregnancy. Vascular catheters were surgically inserted in 17 pregnant ewes and their fetuses at 103–120 d gestation (term = 147 ± 3 d). Ewes were fed a diet providing either 100% (control; n = 9) or approximately 155% (well fed; n = 8) of the maintenance energy requirements and fetal perirenal and interscapular fat depots were collected at 139–141 d gestation. There was a significant relationship between the relative mass of fetal unilocular fat and fetal glucose (relative mass of unilocular fat, 1.14; fetal glucose, +0.16; r = 0.50; P < 0.04; n = 17), but not insulin, concentrations in the control and well-fed groups. In contrast to the controls, there was a positive relationship between the relative abundance of leptin mRNA and fetal insulin, but not glucose, concentrations in fetal perirenal adipose tissue in the well-fed group. A moderate increase in maternal nutrition also resulted in a strong reciprocal relationship between uncoupling protein 1 and leptin expression in fetal perirenal adipose tissue in late gestation (well-fed group: uncoupling protein 1 mRNA:18S rRNA, -0.51; leptin mRNA:ß-actin mRNA, +1.53; r = 0.80; P < 0.02; n = 8). These studies provide evidence that fetal glucose and insulin differentially regulate fetal fat deposition and leptin mRNA expression within the fetal perirenal fat depot in the well nourished animal during late gestation.
|Item Type:||Article (Commonwealth Reporting Category C)|
|Additional Information:||Author version not held.|
|Uncontrolled Keywords:||leptin synthesis; fetus; fetal glucose|
|Fields of Research (FOR2008):||11 Medical and Health Sciences > 1199 Other Medical and Health Sciences > 119999 Medical and Health Sciences not elsewhere classified|
06 Biological Sciences > 0606 Physiology > 060699 Physiology not elsewhere classified
11 Medical and Health Sciences > 1116 Medical Physiology > 111699 Medical Physiology not elsewhere classified
|Socio-Economic Objective (SEO2008):||E Expanding Knowledge > 97 Expanding Knowledge > 970111 Expanding Knowledge in the Medical and Health Sciences|
|Deposited On:||09 May 2010 21:56|
|Last Modified:||18 Sep 2012 12:44|
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