Host species-specific translocation of Escherichia coli

Katouli, M. and Ramos, N. L. and Nettelbladt, C. G. and Ljungdahl, M. and Robinson, W. and Ison, H. M. and Brauner, A. and Mollby, R. (2009) Host species-specific translocation of Escherichia coli. European Journal of Clinical Microbiology and Infectious Diseases, 28 (9). pp. 1095-1103. ISSN 0934-9723

Abstract

The purpose of this paper is to investigate the rate of translocation of Escherichia coli strains in different experimental/animal models. Four proficient translocating E. coli strains isolated from mesenteric lymph nodes (MLNs) and/or the blood of rats (strains KIC-1 and KIC- 2), from a fatal case of pancreatitis (HMLN-1) and from pigs (PC-1 isolated in this study) were tested for their ability to translocate across two host species and the Caco-2 cell line as a model of the human gut epithelium. HMLN-1 was found in the MLNs of all 15 pigs tested. This strain, however, did not translocate in any rats and only colonised the caecum of four rats in small numbers. HMLN-1 and PC-1 were the dominant translocating strains in Caco-2 cells compared to KIC-1 and KIC-2, which were found to translocate at a lower rate in pigs and in Caco-2 cells. The rate of translocation of PC-1 in rats was also very low compared to KIC-1 and KIC-2. We suggest that, in studies aiming to investigate the mechanism of translocation of E. coli strains isolated from humans, rats may not be an appropriate animal model and that the Caco-2 cells or pigs are more suitable in vitro and in vivo models, respectively.


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Item Type: Article (Commonwealth Reporting Category C)
Refereed: Yes
Item Status: Live Archive
Additional Information: © 2009 Springer-Verlag. Awaiting author's version, which may be deposited in accordance with the copyright policy of the publisher.
Depositing User: Ms Helen Ison
Faculty / Department / School: Historic - Faculty of Sciences - Department of Biological and Physical Sciences
Date Deposited: 21 May 2010 11:16
Last Modified: 16 Oct 2014 01:25
Uncontrolled Keywords: Escherichia coli; E. coli; translocation; bacterial typing techniques
Fields of Research (FOR2008): 06 Biological Sciences > 0605 Microbiology > 060501 Bacteriology
11 Medical and Health Sciences > 1103 Clinical Sciences > 110307 Gastroenterology and Hepatology
06 Biological Sciences > 0605 Microbiology > 060502 Infectious Agents
Socio-Economic Objective (SEO2008): E Expanding Knowledge > 97 Expanding Knowledge > 970111 Expanding Knowledge in the Medical and Health Sciences
Identification Number or DOI: doi: 10.1007/s10096-009-0754-0
URI: http://eprints.usq.edu.au/id/eprint/7710

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