Molecular identification of a mycorrhizal Sebacinaceae from the endangered Caladenia atroclavia (black clubbed spider orchid)

Dearnaley, J. D. W. and Murray, A. J. and Mathieson, M. T. (2009) Molecular identification of a mycorrhizal Sebacinaceae from the endangered Caladenia atroclavia (black clubbed spider orchid). Australasian Mycologist, 28 (2-3). pp. 45-50. ISSN 1441-5526

Abstract

Caladenia atroclavia D. Jones & M. Clements is an endangered orchid found only in the Stanthorpe region of south east Queensland. As part of a recovery process for the species we aimed to isolate and identify the mycorrhizal fungus from the plant. Fungal coils were isolated from the orchid and grown into pure culture. DNA was extracted from these cultures and also from colonised plant stem collar regions. Following fungal ITS-DNA PCR amplification, cloning and BLAST similarity searches, we identified the main fungal endophyte within the orchid as a Sebacinaceae sp. Co-inoculation trials showed that isolates of the fungus were capable of germinating seed of the orchid. Neighbour-joining phylogenetic analysis of the ITS-DNA of this mycorrhizal fungus showed that it was related to Sebacinaceae spp. that have been isolated from other Australian Caladenia spp. This fungus will be used in ex situ propagation procedures to ultimately increase the number of plants in the natural state


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Item Type: Article (Commonwealth Reporting Category C)
Refereed: Yes
Item Status: Live Archive
Additional Information: Deposited with blanket permission of publisher.
Depositing User: Ms Leslie Blay
Faculty / Department / School: Historic - Faculty of Sciences - No Department
Date Deposited: 14 Jul 2010 12:15
Last Modified: 27 Sep 2013 02:53
Uncontrolled Keywords: endangered plants; Stanthorpe; fungus; black clubbed spider orchid
Fields of Research (FOR2008): 06 Biological Sciences > 0607 Plant Biology > 060702 Plant Cell and Molecular Biology
06 Biological Sciences > 0605 Microbiology > 060505 Mycology
05 Environmental Sciences > 0501 Ecological Applications > 050104 Landscape Ecology
Socio-Economic Objective (SEO2008): D Environment > 96 Environment > 9613 Remnant Vegetation and Protected Conservation Areas > 961308 Remnant Vegetation and Protected Conservation Areas at Regional or Larger Scales
URI: http://eprints.usq.edu.au/id/eprint/7671

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