Saprolegniaceae identified on amphibian eggs throughout the Pacific Northwest,USA, by internal transcribed spacer sequences and phylogenetic analysis

Petrisko, Jill E. and Pearl, Christopher A. and Pilliod, David S. and Sheridan, Peter P. and Williams, Charles F. and Peterson, Charles R. and Bury, R. Bruce (2008) Saprolegniaceae identified on amphibian eggs throughout the Pacific Northwest,USA, by internal transcribed spacer sequences and phylogenetic analysis. Mycologia, 100 (2). pp. 171-180. ISSN 0027-5514

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Abstract

We assessed the diversity and phylogeny of Saprolegniaceae on amphibian eggs from the Pacific Northwest, with particular focus on Saprolegnia ferax, a species implicated in high egg mortality. We identified isolates from eggs of six amphibians with the internal transcribed spacer (ITS) and 5.8S gene regions and BLAST of the GenBank database. We identified 68 sequences as Saprolegniaceae and 43 sequences as true fungi from at least nine genera. Our phylogenetic analysis of the Saprolegniaceae included isolates within the genera Saprolegnia, Achlya and Leptolegnia. Our phylogeny grouped S. semihypogyna with Achlya rather than with the Saprolegnia reference sequences. We found only one isolate that grouped closely with S. ferax, and this came from a hatchery-raised salmon (Idaho) that we sampled opportunistically. We had representatives of 7-12 species and three genera of Saprolegniaceae on our amphibian eggs. Further work on the ecological roles of different species of Saprolegniaceae is needed to clarify their potential importance in amphibian egg mortality and potential links to population declines.


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Item Type: Article (Commonwealth Reporting Category C)
Refereed: Yes
Item Status: Live Archive
Additional Information: Deposited in accordance with copyright conditions of publisher (Mycological Society of America)(See Sherpa Romeo website http://www.sherpa.ac.uk/romeo/index.php)
Depositing User: Dr Jill Petrisko
Faculty / Department / School: Historic - Faculty of Sciences - Department of Biological and Physical Sciences
Date Deposited: 26 Mar 2010 06:47
Last Modified: 04 Aug 2014 19:22
Uncontrolled Keywords: achlya, amphibian decline, egg, lake, Leptolegnia, oomycete, Saprolegnia ferax, S. semihypogyna
Fields of Research (FOR2008): 06 Biological Sciences > 0605 Microbiology > 060504 Microbial Ecology
Socio-Economic Objective (SEO2008): E Expanding Knowledge > 97 Expanding Knowledge > 970106 Expanding Knowledge in the Biological Sciences
Identification Number or DOI: doi: 10.3852/mycologia.100.2.171
URI: http://eprints.usq.edu.au/id/eprint/7135

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