A novel and simple method for genotyping the mdx mouse using high-resolution melt polymerase chain reaction

Trebbin, Andrea L. and Hoey, Andrew J. (2009) A novel and simple method for genotyping the mdx mouse using high-resolution melt polymerase chain reaction. Muscle and Nerve, 39 (5). pp. 603-608. ISSN 0148-639X

Abstract

The mdx mouse mutation arises from a C-to-T point mutation, which terminates the translation of dystrophin and results in the loss of a functional dystrophin protein. mdx mice are used widely in studies of the role of dystrophon and of potential treatments for Duchenne muscular dystrophy, thus accurate genotyping is essential. Current methods require labor-intensive efforts and can often lead to misconstrued results. This study describes a simple and highly reliable, sensitive, and user-friendly, high-resolution melt (HRM) assay that is able to utilize DNA obtained from a variety of sources in order to genotype the known sequence variant of the mdx mouse.


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Item Type: Article (Commonwealth Reporting Category C)
Refereed: Yes
Item Status: Live Archive
Additional Information: Awaiting Author's version which may be deposited in accordance with the copyright policy of the publisher.
Depositing User: Ms Debbie White
Faculty / Department / School: Historic - Faculty of Sciences - Department of Biological and Physical Sciences
Date Deposited: 24 Feb 2010 12:08
Last Modified: 02 Jul 2013 23:33
Uncontrolled Keywords: Duchenne muscular dystrophy (DMD); genotyping; high-resolution melting (HRM); mdx mutation; single-nucleotide polymorphism (SNP)
Fields of Research (FOR2008): 06 Biological Sciences > 0699 Other Biological Sciences > 069999 Biological Sciences not elsewhere classified
06 Biological Sciences > 0604 Genetics > 060403 Developmental Genetics (incl. Sex Determination)
11 Medical and Health Sciences > 1103 Clinical Sciences > 110311 Medical Genetics (excl. Cancer Genetics)
Socio-Economic Objective (SEO2008): E Expanding Knowledge > 97 Expanding Knowledge > 970106 Expanding Knowledge in the Biological Sciences
E Expanding Knowledge > 97 Expanding Knowledge > 970111 Expanding Knowledge in the Medical and Health Sciences
Identification Number or DOI: doi: 10.1002/mus.21215
URI: http://eprints.usq.edu.au/id/eprint/6408

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