The use of high resolution melting (HRM) to map single nucleotide polymorphism markers linked to a covered smut resistance gene in barley

Lehmensiek, Anke and Sutherland, Mark W. and McNamara, Raymond B. (2008) The use of high resolution melting (HRM) to map single nucleotide polymorphism markers linked to a covered smut resistance gene in barley. Theoretical and Applied Genetics, 117 (5). pp. 721-728. ISSN 0040-5752

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Using an established genetic map, a single gene conditioning covered smut resistance, Ruh.7H, was mapped to the telomere region of chromosome 7HS in an Alexis/ Sloop doubled haploid barley population. The closest marker to Ruh.7H, abg704 was 7.5 cM away. Thirteen loci on the distal end of 7HS with potential to contain single nucleotide polymorphisms (SNPs) were identiWed by applying a comparative genomics approach using rice sequence data. Of these, one locus produced polymorphic co-dominant bands of diVerent size while two further loci contained SNPs that were identiWed using the recently developed high resolution melting (HRM) technique. Two of these markers Xanked Ruh.7H with the proximal marker located 3.8 cM and the distal marker 2.7 cM away. This is the Wrst report on the application of the HRM technique to SNP detection and to rapid scoring of known cleaved ampliWed polymorphic sequence (CAPS) markers in plants. This simple, precise post-PCR technique should Wnd widespread use in the Wne-mapping of genetic regions of interest in complex cereal and other plant genomes.

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Item Type: Article (Commonwealth Reporting Category C)
Refereed: Yes
Item Status: Live Archive
Additional Information: Author's version deposited in accordance with the copyright policy of the publisher.
Depositing User: Mrs Julie-Anne Christensen
Faculty / Department / School: Historic - Faculty of Sciences - Department of Biological and Physical Sciences
Date Deposited: 06 Oct 2009 02:31
Last Modified: 26 Sep 2013 00:03
Uncontrolled Keywords: single gene; smut resistance; haploid barley
Fields of Research (FOR2008): 06 Biological Sciences > 0604 Genetics > 060408 Genomics
06 Biological Sciences > 0607 Plant Biology > 060704 Plant Pathology
07 Agricultural and Veterinary Sciences > 0703 Crop and Pasture Production > 070308 Crop and Pasture Protection (Pests, Diseases and Weeds)
Socio-Economic Objective (SEO2008): B Economic Development > 82 Plant Production and Plant Primary Products > 8205 Winter Grains and Oilseeds > 820501 Barley
Identification Number or DOI: doi: 10.1007/s00122-008-0813-4

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