The use of high resolution melting (HRM) to map single nucleotide polymorphism markers linked to a covered smut resistance gene in barley

Lehmensiek, Anke and Sutherland, Mark W. and McNamara, Raymond B. (2008) The use of high resolution melting (HRM) to map single nucleotide polymorphism markers linked to a covered smut resistance gene in barley. Theoretical and Applied Genetics, 117 (5). pp. 721-728. ISSN 0040-5752

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Using an established genetic map, a single gene
conditioning covered smut resistance, Ruh.7H, was mapped
to the telomere region of chromosome 7HS in an Alexis/
Sloop doubled haploid barley population. The closest
marker to Ruh.7H, abg704 was 7.5 cM away. Thirteen loci
on the distal end of 7HS with potential to contain single
nucleotide polymorphisms (SNPs) were identiWed by
applying a comparative genomics approach using rice
sequence data. Of these, one locus produced polymorphic
co-dominant bands of diVerent size while two further loci
contained SNPs that were identiWed using the recently
developed high resolution melting (HRM) technique. Two
of these markers Xanked Ruh.7H with the proximal marker
located 3.8 cM and the distal marker 2.7 cM away. This is
the Wrst report on the application of the HRM technique to
SNP detection and to rapid scoring of known cleaved
ampliWed polymorphic sequence (CAPS) markers in plants.
This simple, precise post-PCR technique should Wnd widespread
use in the Wne-mapping of genetic regions of interest
in complex cereal and other plant genomes.

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Item Type: Article (Commonwealth Reporting Category C)
Refereed: Yes
Item Status: Live Archive
Additional Information (displayed to public): Author's version deposited in accordance with the copyright policy of the publisher.
Depositing User: Mrs Julie-Anne Christensen
Faculty / Department / School: Historic - Faculty of Sciences - Department of Biological and Physical Sciences
Date Deposited: 06 Oct 2009 02:31
Last Modified: 26 Sep 2013 00:03
Uncontrolled Keywords: single gene; smut resistance; haploid barley
Fields of Research (FoR): 06 Biological Sciences > 0604 Genetics > 060408 Genomics
06 Biological Sciences > 0607 Plant Biology > 060704 Plant Pathology
07 Agricultural and Veterinary Sciences > 0703 Crop and Pasture Production > 070308 Crop and Pasture Protection (Pests, Diseases and Weeds)
Socio-Economic Objective (SEO): B Economic Development > 82 Plant Production and Plant Primary Products > 8205 Winter Grains and Oilseeds > 820501 Barley
Identification Number or DOI: doi: 10.1007/s00122-008-0813-4

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