A RAPD-PCR genotyping assay which correlates with serotypes of group B streptococci

Zhang, G. W. and Kotiw, M. and Daggard, G. (2002) A RAPD-PCR genotyping assay which correlates with serotypes of group B streptococci. Letters in Applied Microbiology, 35 (3). pp. 247-250. ISSN 0266-8254

Abstract

Aims: The purpose of this study was to determine if DNA polymorphisms generated by RAPD-PCR could be used to characterize Group B streptococci (GBS) for epidemiological purposes. Methods and Results: 30 unrelated, previously serotyped strains were analysed by RAPD-PCR using two 10-mer primers (5' TGCGAGAGTC 3' and 5' AGAGGGCACA 3'). Both primers generated DNA electropherotype patterns which, on analysis, clustered the isolates within their respective serotypes. A blind test of a further 3 field isolates also defined these strains within their subsequently determined serotypes. The detection of DNA polymorphisms between isolates within a serotype confirmed previous reports of the heterogenous nature of individual GBS serotypes. Conclusions: The RAPD-PCR is a potentially useful assay for the rapid characterization of neonatal infections associated with group B streptococci. The method appears to be more discriminatory than conventional serological assays. Significance and Impact of the Study: The RAPD-PCR assay is faster, more convenient and easier to perform than alternative DNA analytical procedures such as Pulsfield Gel Electrophoresis. We were able to reproduce the same results following re-testing of all isolates some 12 months later which suggests that the assay may be robust enough for use in routine epidemiological investigations.


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Item Type: Article (Commonwealth Reporting Category C)
Refereed: Yes
Item Status: Live Archive
Additional Information: Deposited in accordance with the copyright policy of the publisher.
Depositing User: epEditor USQ
Faculty / Department / School: Historic - Faculty of Sciences - Department of Biological and Physical Sciences
Date Deposited: 20 Nov 2007 00:01
Last Modified: 02 Jul 2013 22:54
Uncontrolled Keywords: group B streptococci; bacterial typing techniques; DNA; genotype; random amplified polymorphic DNA technique; sequence analysis; serotyping; streptococcal infections; streptococcus agalactiae
Fields of Research (FOR2008): 11 Medical and Health Sciences > 1107 Immunology > 110706 Immunogenetics (incl. Genetic Immunology)
06 Biological Sciences > 0605 Microbiology > 060501 Bacteriology
11 Medical and Health Sciences > 1114 Paediatrics and Reproductive Medicine > 111403 Paediatrics
Socio-Economic Objective (SEO2008): C Society > 92 Health > 9201 Clinical Health (Organs, Diseases and Abnormal Conditions) > 920109 Infectious Diseases
Identification Number or DOI: doi: 10.1046/j.1472-765X.2002.01177.x
URI: http://eprints.usq.edu.au/id/eprint/3546

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