Production of reactive oxygen species during non-specific elicitation, non-host resistance and field resistance expression in cultured tobacco cells

Able, Amanda J. and Sutherland, Mark W. and Guest, David I. (2003) Production of reactive oxygen species during non-specific elicitation, non-host resistance and field resistance expression in cultured tobacco cells. Functional Plant Biology, 30 (1). pp. 91-99. ISSN 1445-4408

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Abstract

[Abstract]: We examined production of reactive oxygen species (ROS) and induction of cell death in tissue-cultured tobacco cells undergoing different disease resistance responses. A superoxide-dependent hypersensitive response occurs during both the race-specific resistance response of tobacco cells challenged with incompatible zoospores of Phytophthora nicotianae and during non-specific elicitation of tobacco cells challenged with Phytophthora glucan elicitors extracted from the fungal cell wall. Inhibition studies are consistent with dependence upon endogenous Ca2+ levels, and with involvement of NAD(P)H oxidase and peroxidases in production of ROS during both specific and non-specific elicitation. The patterns of resistance expression during non-host resistance or field resistance responses appear to be similar to race-specific resistance expression with regard to the timing and order of events. However, the intensity of the response is very much reduced. In contrast, during non-specific elicitation, these temporal patterns are significantly altered. The differences in timing, intensity and extent of responses during different modes of disease resistance expression indicate that stimulation of cultured plant cells with non-specific soluble fractions in order to model in planta events during plant / Oomycete and, by implication, plant / fungal interactions, has significant limitations.


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Item Type: Article (Commonwealth Reporting Category C)
Refereed: Yes
Item Status: Live Archive
Additional Information: Deposited in accordance with the copyright policy of the publisher. Pre-print version of article, as made available here, differs in title from the Published version. Pre-print title: Reactive oxygen species production during non-specific elicitation, non-host resistance and field resistance expression in cultured tobacco cells.
Depositing User: epEditor USQ
Faculty / Department / School: Historic - Faculty of Sciences - Department of Biological and Physical Sciences
Date Deposited: 16 Nov 2007 04:26
Last Modified: 28 Oct 2013 04:29
Uncontrolled Keywords: disease resistance, glucan elicitors, plant–pathogen interactions, reactive oxygen species
Fields of Research (FOR2008): 06 Biological Sciences > 0607 Plant Biology > 060704 Plant Pathology
URI: http://eprints.usq.edu.au/id/eprint/3538

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