Laser-based fluorescence microscopy

Learmonth, Robert P. (2002) Laser-based fluorescence microscopy. Microbiology Australia, 23 (1). pp. 16-17. ISSN 1324-4272

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Official URL: http://www.theasm.com.au/publications

Abstract

[Introduction]:Many microbiologists will be familiar with transmission fluorescence microscopy as well the improved epifluorescence microscopy. However, microbiologists are generally less familiar with confocal laser scanning microscopy (CLSM), despite its use since the early 1980s in cell biology and related fields. Furthermore, the new technique of multi-photon laser scanning microscopy (MPLSM) is not yet widely appreciated. This article briefly outlines some principles and applications of these two relatively recent microscopic techniques. With their inherent advantages, CLSM and MPLSM could be widely applied to enhance microbiological studies.

Item Type:Article (Commonwealth Reporting Category C)
Additional Information:Awaiting copyright advice. ©2002 The Australian Society for Microbiology Inc. All rights reserved. No part of this publication may be reproduced or copied in any form or by any means without the written permission of the Australian Society for Microbiology.
Uncontrolled Keywords:fluorescence, laser, scanning, microscopy, confocal, multi-photon
Fields of Research (FOR2008):02 Physical Sciences > 0205 Optical Physics > 020503 Nonlinear Optics and Spectroscopy
02 Physical Sciences > 0205 Optical Physics > 020502 Lasers and Quantum Electronics
06 Biological Sciences > 0605 Microbiology > 060599 Microbiology not elsewhere classified
Subjects:270000 Biological Sciences > 270300 Microbiology
240000 Physical Sciences > 249900 Other Physical Sciences > 249903 Instruments and Techniques
240000 Physical Sciences > 249900 Other Physical Sciences > 249901 Biophysics
270000 Biological Sciences > 270100 Biochemistry and Cell Biology
Socio-Economic Objective (SEO2008):UNSPECIFIED
ID Code:3346
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Deposited On:04 Oct 2007
Last Modified:17 Mar 2010 13:47

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