Able, Amanda J. and Guest, David I. and Sutherland, Mark W. (2001) Relationship between transembrane ion movements, production of reactive oxygen species and the hypersensitive response during the challenge of tobacco suspension cells by zoospores of Phytophthora nicotianae. Physiological and Molecular Plant Pathology, 58 (5). pp. 189-198. ISSN 0885-5765
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Official URL: http://dx.doi.org/10.1006/pmpp.2001.032
Identification Number or DOI: doi: 10.1006/pmpp.2001.032
We have examined the responses of suspension cultured tobacco cells after inoculation with zoospores from compatible or incompatible races of the Oomycete pathogen Phytophthora nicotianae. The incompatible interaction, characterized by the production of reactive oxygen species (ROS) (speci®cally superoxide (HO2=Oÿ2 ) and hydrogen peroxide (H2O2)), and by a hypersensitive response (HR), was not dependent upon the presence of exogenous calcium. However, perturbation of calcium ion movements by EGTA or LaCl3 suppressed both ROS production and the extent of cell death. The Ca2-speci®c ionophore, A23187, slightly enhanced ROS production during the incompatible interaction but had no eect on the responses of susceptible or unchallenged cells. These results con®rm that both ROS production and the HR are potentiated by movement of endogenous calcium across the plasmalemma. While there was no evidence of a transmembrane K/H exchange immediately following incompatible zoospore challenge, a later potassium eux from cells coincided with the onset of the HR. Unexpectedly, medium pH drifted downwards during all host cell responses suggesting that alkaline peroxidases are not the major source of ROS generation during an incompatible tobacco cell/zoospore interaction.
|Item Type:||Article (Commonwealth Reporting Category C)|
|Additional Information:||Author's version unavailable in electronic format.|
|Uncontrolled Keywords:||calcium, hydrogen peroxide, HR, Nicotiana tabacum, Phytophthora nicotianae, plant-, pathogen interactions, ROS, superoxide, XTT, potassium, pH|
|Fields of Research (FOR2008):||06 Biological Sciences > 0607 Plant Biology > 060704 Plant Pathology|
|Subjects:||270000 Biological Sciences > 270400 Botany > 270403 Plant Pathology|
|Socio-Economic Objective (SEO2008):||UNSPECIFIED|
|Deposited On:||18 Jul 2008 09:58|
|Last Modified:||03 Jun 2011 11:21|
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