Able, Amanda J. and Guest, David I. and Sutherland, Mark W. (2000) Hydrogen peroxide yields during the incompatible interaction of tobacco suspension cells inoculated with Phytophthora nicotiannae. Plant Physiology, 124 (2). pp. 899-910. ISSN 1532-2548
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Official URL: http://www.plantphysiol.org/cgi/reprint/124/2/899
Rates of H2O2 production by tobacco suspension cells inoculated with zoospores from compatible or incompatible races of the pathogen Phytophthora nicotianae were followed by direct measurement of oxygen evolution from culture supernatants following catalase addition. Rates of HO2./O2- production were compared by following the formation of the formazan of sodium, 3'-[1-[phenylamino-carbonyl]-3,4-tetrazolium]-bis(4-methoxy-6-nitro) benzene-sulfonic acid hydrate. In the incompatible interaction only, both reactive oxygen species (ROS) were produced by the cultured host cells in a minor burst between 0 and 2 h and then in a major burst between 8 and 12 h after inoculation. Absolute levels of H2O2 could not be accurately measured due to its metabolism by host cells, but results are consistent with the majority of H2O2 being formed via dismutation of HO2./O2-. The effects of inhibitors of endogenous Cu/Zn superoxide dismutase (diethyldithiocarbamate) and catalase (3-amino-1,2,4-triazole and salicylic acid) were also examined. Yields of ROS in the presence of the inhibitors diphenylene iodonium, allopurinol, and salicylhydroxamic acid suggest that ROS were generated in incompatible host responses by more than one mechanism.
|Item Type:||Article (Commonwealth Reporting Category C)|
|Additional Information:||Author's version unavilable in electronic format.|
|Uncontrolled Keywords:||hydrogen peroxide, Phytophthora nicotianae|
|Fields of Research (FOR2008):||06 Biological Sciences > 0607 Plant Biology > 060704 Plant Pathology|
07 Agricultural and Veterinary Sciences > 0703 Crop and Pasture Production > 070304 Crop and Pasture Biomass and Bioproducts
|Subjects:||210000 Science - General|
270000 Biological Sciences > 270400 Botany > 270403 Plant Pathology
|Socio-Economic Objective (SEO2008):||UNSPECIFIED|
|Deposited On:||28 Jul 2008 16:52|
|Last Modified:||20 Jun 2012 11:33|
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