Improving the efficiency of haploid wheat production mediated by wide crossing

Kammholz, Stephen J. and Sutherland, Mark W. and Banks, Phillip (1996) Improving the efficiency of haploid wheat production mediated by wide crossing. Sabrao Journal, 28. pp. 37-46. ISSN 1324-4612

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[Summary]: This study examined several possible means of improving the wheat (Triticum aestivum L.) x maize (Zea mays L.) method for haploid production in Australian hard white spring wheat genotypes. Two wheat varieties were pollinated by each of five maize lines, giving an average of 22 embryos per 100 florets. One maize inbred gave a significantly lower haploid embryo yield. Embryos rescued 12 or 15 days post pollination had a significantly higher germination percentage (51 to
80%) than embryos rescued 9, 17, 19 or 21 days after pollination (6.4 to 31%).
Embryos 12 to 15 days old were found to be large and easily rescued. Haploid wheat embryos germinated at equal frequencies on four commercial tissue culture media and
germination frequency was not affected by differing levels of 6-benzylarninopurine (BAP). However, the rate at which haploid embryos germinated was found to be significantly affected by both the type of medium and the Cytokinin level. Haploid embryos germinated fastest on an MS based medium with 0.05 mg/L BAP.

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Item Type: Article (Commonwealth Reporting Category C)
Refereed: No
Item Status: Live Archive
Additional Information (displayed to public): No evidence of copyright restrictions.
Depositing User: Prof Mark Sutherland
Faculty / Department / School: Historic - Faculty of Sciences - Department of Biological and Physical Sciences
Date Deposited: 31 Jul 2008 02:29
Last Modified: 09 Feb 2015 04:50
Uncontrolled Keywords: wheat, maize, haploid, embryo culture, doubled haploid
Fields of Research (FoR): 06 Biological Sciences > 0607 Plant Biology > 060705 Plant Physiology
06 Biological Sciences > 0604 Genetics > 060499 Genetics not elsewhere classified
07 Agricultural and Veterinary Sciences > 0703 Crop and Pasture Production > 070305 Crop and Pasture Improvement (Selection and Breeding)

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