Expression of a polygalacturonase enzyme in germinating pollen of Brassica napus

Dearnaley, John and Daggard, Grant (2001) Expression of a polygalacturonase enzyme in germinating pollen of Brassica napus. Sexual Plant Reproduction, 13 (5). pp. 265-271. ISSN 0934-0882

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Abstract

[Abstract]: Penetration of pollen tubes through stigmatic tissues in Brassica napus L. may involve the release of cell wall modifying enzymes from the pollen tube tip. We have examined the expression of a pectin-degrading polygalacturonase (PG) enzyme in unpollinated and early and late pollinated stigmas via immunoblotting and immuno light microscopy using a PG polyclonal antibody. Immunoblotting analysis indicated that PG enzyme was present at low levels in unpollinated stigmas and at high levels in pollinated stigmas. The level of PG did not detectably increase between early and late pollinated stigmas. Immuno light microscopy demonstrated that PG enzyme was present in ungerminated pollen grains, stigmatic papillae and in the tip of pollen tubes growing into the papillar wall. This latter evidence suggests that PG enzyme may play an important role in papillar cell wall penetration during pollination although other interpretations of the role of pollen PG should not be discounted.


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Item Type: Article (Commonwealth Reporting Category C)
Refereed: Yes
Item Status: Live Archive
Additional Information: Deposited in accordance with the copyright policy of the publisher.
Depositing User: Ms Debbie White
Faculty / Department / School: Historic - Faculty of Sciences - Department of Biological and Physical Sciences
Date Deposited: 11 Oct 2007 01:16
Last Modified: 02 Jul 2013 22:47
Uncontrolled Keywords: Brassica napus, pollen tubes, stigma, pollination, polygalacturonase
Fields of Research (FOR2008): 06 Biological Sciences > 0607 Plant Biology > 060702 Plant Cell and Molecular Biology
06 Biological Sciences > 0607 Plant Biology > 060799 Plant Biology not elsewhere classified
06 Biological Sciences > 0607 Plant Biology > 060703 Plant Developmental and Reproductive Biology
Identification Number or DOI: doi: 10.1007/s004970000062
URI: http://eprints.usq.edu.au/id/eprint/2884

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