Evaluation of the immunogenicity of the P97R1 adhesin of Mycoplasma hyopnemoniae as a mucosal vaccine in mice

Chen, Austen Y. and Fry, Scott R. and Forbes-Faulkner, Judy and Daggard, Grant and Mukkur, T. K. S. (2006) Evaluation of the immunogenicity of the P97R1 adhesin of Mycoplasma hyopnemoniae as a mucosal vaccine in mice. Journal of Medical Microbiology, 55 (7). pp. 923-929. ISSN 0022-2615

Abstract

The immunogenicity of P97 adhesin repeat region R1 (P97R1) of Mycoplasma hyopneumoniae, an important pathogenesis-associated region of P97, was evaluated in mice as a mucosal vaccine. Mice were immunized orally with attenuated Salmonella typhimurium aroA strain CS332 harbouring a eukaryotic or prokaryotic expression vector encoding P97R1. Local and systemic immune responses were analysed by ELISA on mouse sera, lung washes and splenocyte supernatants following splenocyte stimulation with specific antigens in vitro. Although no P97R1-specific antibody responses were detected in serum and lung washes, significant gamma interferon was produced by P97R1-stimulated splenocytes from mice immunized orally with S. typhimurium aroA harbouring either expression system, indicating induction of a cell-mediated immune response. These results suggested that live bacterial vectors carrying DNA vaccines or expressing heterologous antigens preferentially induce a Th1 response. Surprisingly, however, mice immunized with the vaccine carrier S. typhimurium aroA CS332 induced serum IgG, but not mucosal IgA, against P97R1 or S. typhimurium aroA CS332 whole-cell lysate, emphasizing the importance of assessing the suitability of attenuated S. typhimurium antigen-carrier delivery vectors in the mouse model prior to their evaluation as potential vaccines in the target species, which in this instance was pigs.


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Item Type: Article (Commonwealth Reporting Category C)
Refereed: Yes
Item Status: Live Archive
Additional Information: Awaiting Author's version from author. 'This is an author manuscript that has been accepted for publication in Journal of Medical Microbiology, copyright Society for General Microbiology, but has not been copy-edited, formatted or proofed. Cite this article as appearing in Journal of Medical Microbiology. This version of the manuscript may not be duplicated or reproduced, other than for personal use or within the rule of ‘Fair Use of Copyrighted Materials’ (section 17, Title 17, US Code), without permission from the copyright owner, Society for General Microbiology. The Society for General Microbiology disclaims any responsibility or liability for errors or omissions in this version of the manuscript or in any version derived from it by any other parties. The final copy-edited, published article, which is the version of record, can be found at http://jmm.sgmjournals.org, and is freely available without a subscription.'
Depositing User: Ms Debbie White
Faculty / Department / School: Historic - Faculty of Sciences - Department of Biological and Physical Sciences
Date Deposited: 11 Oct 2007 01:02
Last Modified: 02 Jul 2013 22:43
Uncontrolled Keywords: AP; alkaline phosphatase; IFN-{gamma}; gamma interferon; P97R1; P97 repeat region R1; PEP; porcine enzootic pneumonia
Fields of Research (FOR2008): 07 Agricultural and Veterinary Sciences > 0707 Veterinary Sciences > 070705 Veterinary Immunology
06 Biological Sciences > 0605 Microbiology > 060599 Microbiology not elsewhere classified
Identification Number or DOI: doi: 10.1099/jmm.0.46088-0
URI: http://eprints.usq.edu.au/id/eprint/2296

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