Resistance gene analogues of wheat: molecular genetic analysis of ESTs

McFadden, H. G. and Lehmensiek, A. and Lagudah, E. S. (2006) Resistance gene analogues of wheat: molecular genetic analysis of ESTs. Theoretical and Applied Genetics, 113 (6). pp. 987-1002. ISSN 0040-5752

Abstract

Using two divergent nucleotide binding site (NBS) regions from wheat sequences of the NBS-LRR (leucine rich repeat) class, we retrieved 211 wheat and barley NBS-containing resistance gene analogue (RGA) expressed sequence tags (ESTs). These ESTs were grouped into 129 gene sequence groups that contained ESTs that were at least 70% identical at the DNA level over at least 200 bp. Probes were obtained for 89 of these RGA families and chromosome locations were determined for 72 of these probes using nullitetrasomic Chinese Spring wheat lines. RFLP analysis of 49 of these RGA probes revealed 65 mappable polymorphic bands in the doubled haploid Cranbrook x Halberd wheat population (C x H). These bands mapped to 49 loci in C £ H. RGA loci were detected on all 21 chromosomes using the nullitetrasomic lines and on 18 chromosomes (linkage groups) in the C x H map. This identified a set of potential markers that could be developed further for use in mapping and ultimately cloning NBS-LRR-type disease resistance genes in wheat.


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Item Type: Article (Commonwealth Reporting Category C)
Refereed: Yes
Item Status: Live Archive
Additional Information: © 2006 Springer-Verlag. Electronic Supplementary Material is available to authorised users in the online version of this article at http://www.dx.doi.org/10.1007/s00122-006-0358-3
Depositing User: Ms Debbie White
Faculty / Department / School: Historic - Faculty of Sciences - Department of Biological and Physical Sciences
Date Deposited: 11 Oct 2007 01:01
Last Modified: 17 Oct 2013 05:35
Uncontrolled Keywords: wheat; gene; expressed sequence tags (ESTs); nucleotide; nullitetrasomic
Fields of Research (FOR2008): 10 Technology > 1001 Agricultural Biotechnology > 100105 Genetically Modified Field Crops and Pasture
07 Agricultural and Veterinary Sciences > 0703 Crop and Pasture Production > 070305 Crop and Pasture Improvement (Selection and Breeding)
06 Biological Sciences > 0604 Genetics > 060405 Gene Expression (incl. Microarray and other genome-wide approaches)
Socio-Economic Objective (SEO2008): B Economic Development > 82 Plant Production and Plant Primary Products > 8205 Winter Grains and Oilseeds > 820599 Winter Grains and Oilseeds not elsewhere classified
Identification Number or DOI: doi: 10.1007/s00122-006-0358-3
URI: http://eprints.usq.edu.au/id/eprint/2294

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