Identifying the optimum process parameters for ultrasonic cellular disruption of e. coli

Al-Juboori, Raed A. and Yusaf, Talal (2012) Identifying the optimum process parameters for ultrasonic cellular disruption of e. coli. International Journal of Chemical Reactor Engineering, 10 (1). pp. 1-32. ISSN 1542-6580

Abstract

The aim of this work is to identify the optimum process parameters of sonication, manosonication and thermosonication for deactivating E. coli ATCC 25922 in water-based suspension. The influence of ultrasonic intensity, frequency, pressure, temperature and treatment time on the efficiency of ultrasonic treatments was investigated in this study theoretically and experimentally. The theoretical part of this study involved solving the Rayleigh-Plesset equation with different parameters and evaluating their effects on the collapse pressure of a bubble with initial radius of 0.01mm. The experimental part of the work was conducted using ultrasonic horn reactor at three levels of intensities; low (17.56 W/cm2), intermediate (21.49 W/cm2) and high (24.17 W/cm2) with fixed frequency. Thermosonication and manosonication experiments were conducted at the sub-lethal temperatures of E. coli; 45, 50, 55 and 60C and pressures of 2, 3 and 4 bars. The optimum treatment conditions of sonication, manosonication and thermosonication were evaluated through calculating the specific energy required to obtain 5 log reduction of E. coli in each treatment. Thermosonication treatment at 21.49W/cm2 and 45C for 4 minutes was found to be the optimum treatment conditions to deactivate E. coli in water-based suspension.


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Item Type: Article (Commonwealth Reporting Category C)
Refereed: Yes
Item Status: Live Archive
Additional Information: Permanent restricted access to published version, due to publisher's copyright policy (de Gruyter)
Depositing User: Mr Raed Ahmed Mahmood
Faculty / Department / School: Historic - Faculty of Engineering and Surveying - Department of Agricultural, Civil and Environmental Engineering
Date Deposited: 30 Jul 2012 03:53
Last Modified: 07 Nov 2013 04:32
Uncontrolled Keywords: ultrasonic horn reactor; sonication; thermosonication; manosonication; E. coli ATCC 25922; Rayleigh-Plesset equation
Fields of Research (FOR2008): 06 Biological Sciences > 0601 Biochemistry and Cell Biology > 060199 Biochemistry and Cell Biology not elsewhere classified
09 Engineering > 0904 Chemical Engineering > 090410 Water Treatment Processes
09 Engineering > 0904 Chemical Engineering > 090409 Wastewater Treatment Processes
09 Engineering > 0907 Environmental Engineering > 090703 Environmental Technologies
Socio-Economic Objective (SEO2008): D Environment > 96 Environment > 9606 Environmental and Natural Resource Evaluation > 960608 Rural Water Evaluation (incl. Water Quality)
Identification Number or DOI: doi: 10.1515/1542-6580.2937
URI: http://eprints.usq.edu.au/id/eprint/21596

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