Immunological response to parenteral vaccination with recombinant hepatitis B virus surface antigen virus-like particles expressing Helicobacter pylori KatA epitopes in a murine H. pylori challenge model

Kotiw, Michael and Johnson, Megan and Pandey, Manisha and Fry, Scott and Hazell, Stuart L. and Netter, Hans J. and Good, Michael F. and Olive, Colleen (2012) Immunological response to parenteral vaccination with recombinant hepatitis B virus surface antigen virus-like particles expressing Helicobacter pylori KatA epitopes in a murine H. pylori challenge model. Clinical and Vaccine Immunology, 19 (2). pp. 268-276. ISSN 1556-6811

Abstract

Virus-like particles (VLPs) based on the small envelope protein of hepatitis B virus (HBsAg-S) are immunogenic at the B- and T-cell level. In this study, we inserted overlapping sequences encoding the carboxy terminus of the Helicobacter pylori katA gene product into HBsAg-S. The HBsAg-S–KatA fusion proteins were able to assemble into secretion-competent VLPs (VLP-KatA). The VLP-KatA proteins were able to induce KatA-specific antibodies in immunized mice. The mean total IgG antibody titers 41 days post-primary immunization with VLP-KatA (2.3 × 103) were significantly greater (P < 0.05) than those observed for vaccination with VLP alone (5.2 × 102). Measurement of IgG isotypes revealed responses to both IgG1 and IgG2a (mean titers, 9.0 × 104 and 2.6 × 104, respectively), with the IgG2a response to vaccination with VLP-KatA being significantly higher than that for mice immunized with KatA alone (P < 0.05). Following challenge of mice with H. pylori, a significantly reduced bacterial load in the gastric mucosa was observed (P < 0.05). This is the first report describing the use of VLPs as a delivery vehicle for H. pylori antigens.


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Item Type: Article (Commonwealth Reporting Category C)
Refereed: Yes
Item Status: Live Archive
Additional Information: Published ahead of print 28 December 2011
Depositing User: Assoc Prof Mike Kotiw
Faculty / Department / School: Historic - Faculty of Sciences - Department of Biological and Physical Sciences
Date Deposited: 28 Jul 2012 01:36
Last Modified: 21 Jul 2014 05:40
Uncontrolled Keywords: aluminum hydroxide; bacterial protein; epitope; hepatitis B surface antigen; hybrid protein; immunoglobulin G1 antibody; immunoglobulin G2a antibody; KatA protein; recombinant Hepatitis B virus surface antigen Helicobacter pylori KatA fusion protein virus like particle vaccine; unclassified drug; virus like particle vaccine
Fields of Research (FOR2008): 11 Medical and Health Sciences > 1107 Immunology > 110702 Applied Immunology (incl. Antibody Engineering, Xenotransplantation and T-cell Therapies)
11 Medical and Health Sciences > 1108 Medical Microbiology > 110804 Medical Virology
11 Medical and Health Sciences > 1103 Clinical Sciences > 110307 Gastroenterology and Hepatology
Socio-Economic Objective (SEO2008): E Expanding Knowledge > 97 Expanding Knowledge > 970111 Expanding Knowledge in the Medical and Health Sciences
Identification Number or DOI: doi: 10.1128/​CVI.05295-11
URI: http://eprints.usq.edu.au/id/eprint/21590

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