Knight, N. L. and Sutherland, M. W. (2011) A rapid differential staining technique for Fusarium pseudograminearum in cereal tissues during crown rot infections. Plant Pathology, 60 (6). pp. 1140-1143. ISSN 0032-0862
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Official URL: http://onlinelibrary.wiley.com/doi/10.1111/j.1365-3059.2011.02462.x/pdf
Identification Number or DOI: doi: 10.1111/j.1365-3059.2011.02462.x
Abstract
A novel fluorescence-based differential staining procedure has been developed for the rapid visualization of Fusarium pseudograminearum hyphae in cereal tissues. Infected tissues are stained with safranin, which binds to host cell walls, while hyphal tissues are stained with the fluorescent dye solophenyl flavine 7GFE. The method is rapid, does not require clearing of culm cross-sections, and provides high contrast informative images of fungal and plant structures during pathogenesis.
| Item Type: | Article (Commonwealth Reporting Category C) |
|---|---|
| Additional Information: | Author version deposited in accordance with the copyright policy of the publisher. Copyright 2011 The Authors. Journal compilation Copyright 2011 BSPP. |
| Uncontrolled Keywords: | cereals; fluorescent microscopy; Fusarium pseudograminearum; staining; visualization |
| Fields of Research (FOR2008): | 06 Biological Sciences > 0607 Plant Biology > 060702 Plant Cell and Molecular Biology 06 Biological Sciences > 0605 Microbiology > 060505 Mycology 07 Agricultural and Veterinary Sciences > 0703 Crop and Pasture Production > 070308 Crop and Pasture Protection (Pests, Diseases and Weeds) |
| Subjects: | UNSPECIFIED |
| Socio-Economic Objective (SEO2008): | B Ecomonic Development > 82 Plant Production and Plant Primary Products > 8299 Other Plant Production and Plant Primary Products > 829999 Plant Production and Plant Primary Products not elsewhere classified |
| ID Code: | 21021 |
| Deposited By: | |
| Deposited On: | 25 Mar 2012 14:33 |
| Last Modified: | 05 Jun 2012 09:45 |
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