A rapid differential staining technique for Fusarium pseudograminearum in cereal tissues during crown rot infections

Knight, N. L. and Sutherland, M. W. (2011) A rapid differential staining technique for Fusarium pseudograminearum in cereal tissues during crown rot infections. Plant Pathology, 60 (6). pp. 1140-1143. ISSN 0032-0862

Abstract

A novel fluorescence-based differential staining procedure has been developed for the rapid visualization of Fusarium pseudograminearum hyphae in cereal tissues. Infected tissues are stained with safranin, which binds to host cell walls, while hyphal tissues are stained with the fluorescent dye solophenyl flavine 7GFE. The method is rapid, does not require clearing of culm cross-sections, and provides high contrast informative images of fungal and plant structures during pathogenesis.


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Item Type: Article (Commonwealth Reporting Category C)
Refereed: Yes
Item Status: Live Archive
Additional Information: Author version deposited in accordance with the copyright policy of the publisher. Copyright 2011 The Authors. Journal compilation Copyright 2011 BSPP.
Depositing User: Prof Mark Sutherland
Faculty / Department / School: Historic - Faculty of Sciences - Department of Biological and Physical Sciences
Date Deposited: 25 Mar 2012 04:33
Last Modified: 03 Jul 2013 01:08
Uncontrolled Keywords: cereals; fluorescent microscopy; Fusarium pseudograminearum; staining; visualization
Fields of Research (FOR2008): 06 Biological Sciences > 0607 Plant Biology > 060702 Plant Cell and Molecular Biology
06 Biological Sciences > 0605 Microbiology > 060505 Mycology
07 Agricultural and Veterinary Sciences > 0703 Crop and Pasture Production > 070308 Crop and Pasture Protection (Pests, Diseases and Weeds)
Socio-Economic Objective (SEO2008): B Economic Development > 82 Plant Production and Plant Primary Products > 8299 Other Plant Production and Plant Primary Products > 829999 Plant Production and Plant Primary Products not elsewhere classified
Identification Number or DOI: doi: 10.1111/j.1365-3059.2011.02462.x
URI: http://eprints.usq.edu.au/id/eprint/21021

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