Effects of supplementation with L-proline or inositol on yeast membrane fluidity and ethanol tolerance

Ishmayana, Safri and Kennedy, Ursula and Learmonth, Robert (2011) Effects of supplementation with L-proline or inositol on yeast membrane fluidity and ethanol tolerance. In: 12th Conference on Methods and Applications of Fluorescence Spectroscopy, Imaging and Probes (MAF12), 11-14 Sept 2011, Strasbourg, France.

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Abstract

Fermentation performance of the yeast Saccharomyces cerevisiae in media with high sugar concentration Safri Ishmayana[1,2], Robert P. Learmonth[2], Ursula J. Kennedy[2] [1]Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Padjadjaran, Jln. Raya Bandung-Sumedang km. 21 Jatinangor, West Java, 45363 Indonesia [2]Centre for System Biology, University of Southern Queensland, West St., Toowoomba, QLD 4350 Australia Abstract High sugar concentration is more preferred in industrial bioethanol production, as it can increase the amount of ethanol produced by the end of fermentation. However, when high sugar concentration is used in the media, yeast cells are exposed to high osmotic stress, which can affect the fermentation performance. The present experiment aimed to investigate the fermentation performance of the yeast S. cerevisiae at high sugar concentration, in poor (Yeast nitrogen base/YNB) and rich (yeast extract peptone/YEP) media. Growth parameters and sugar utilization were monitored during the fermentations. The results indicate that all three strains used had stuck fermentations, leaving 50 to 60% residual sugar. Therefore, a follow up experiment was conducted by using media of different nutritional value, including YNB with nitrogen supplementation and YEP, as well as different sugars (sucrose or glucose). Yeast cell grown in YEP had better fermentation performance indicated by higher sugar utilization. Addition of ammonium sulphate to YNB media did not change fermentation performance of the yeast cells. In YEP media, cells grown with glucose tended to maintain better viability than sucrose. Our study confirmed that nutrient availability is very important for fermentation performance. Comparison of YNB and YEP media indicates that nutritionally insufficient media are not suitable for high concentration sugar fermentation.


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Item Type: Conference or Workshop Item (Commonwealth Reporting Category E) (Poster)
Refereed: No
Item Status: Live Archive
Additional Information: This poster paper was not peer-reviewed. Abstract only published.
Depositing User: Assoc Prof Robert Learmonth
Faculty / Department / School: Historic - Faculty of Sciences - Department of Biological and Physical Sciences
Date Deposited: 14 Feb 2012 07:56
Last Modified: 03 Jul 2013 01:00
Uncontrolled Keywords: fermentation performance, high sugar concentration, nutrition availability
Fields of Research (FOR2008): 10 Technology > 1003 Industrial Biotechnology > 100303 Fermentation
Socio-Economic Objective (SEO2008): B Economic Development > 85 Energy > 8505 Renewable Energy > 850599 Renewable Energy not elsewhere classified
E Expanding Knowledge > 97 Expanding Knowledge > 970106 Expanding Knowledge in the Biological Sciences
E Expanding Knowledge > 97 Expanding Knowledge > 970110 Expanding Knowledge in Technology
URI: http://eprints.usq.edu.au/id/eprint/20570

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