Effect of the puroindoline locus and environment on Chinese fresh noodle texture

Storlie, E. and Yang, E. N. and Zou, Y. C. and Chen, D. S. and Sheppard, J. and Martin, D. and Huang, S. and Mares, D. and Sutherland, M. W. (2006) Effect of the puroindoline locus and environment on Chinese fresh noodle texture. Australian Journal of Agricultural Research, 57 (5). pp. 537-542. ISSN 0004-9409

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Abstract

Grain produced from doubled-haploid (DH)wheat lines, developed from a hard- and a soft-grained wheat cultivar, were bulked according to Pinb (puroindoline b) genotypes for an assessment of Chinese fresh noodle texture by a trained taste panel. Each DH line was designated as 'soft' or 'hard' grained, based on a PCR amplification of the wildtype, soft allele, or the mutant, hard allele. Theoretically, the soft and hard grain bulks represented respective Pinb alleles and an independent assortment of unlinked alleles from the parents, Sunco and Chuanyu 12. Grains from the parents and DH lines were grown at 2 locations in Queensland, Australia, and one in Sichuan, China. The grains were milled and processed for a taste panel evaluation in Chengdu, Sichuan. Results suggest the Pinb alleles had a significant effect on noodle softness and explained 30% of the variation; the 'soft' Pinb allele conferred a softer noodle texture. Location had a significant effect on noodle smoothness; wheat grain grown at Biloela, Queensland, produced a smoother noodle texture than grain grown in Sichuan. The effect of location confirms the importance of environment as a variable for this quality character. This investigation exemplifies the utility of Pinb markers for specifically altering Chinese Fresh Noodle texture.


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Item Type: Article (Commonwealth Reporting Category C)
Refereed: Yes
Item Status: Live Archive
Additional Information: Author version deposited in accordance with copyright policy of the publisher. Published version cannot be displayed. See Publisher's link to published version.
Depositing User: Ms Tania Moore
Faculty / Department / School: Historic - Faculty of Sciences - Department of Biological and Physical Sciences
Date Deposited: 31 Mar 2008 13:31
Last Modified: 02 Jul 2013 22:41
Uncontrolled Keywords: Triticum aestivum; grain hardness; bulked segregant analysis; taste panel; Pinb; noodle softness
Fields of Research (FOR2008): 06 Biological Sciences > 0604 Genetics > 060412 Quantitative Genetics (incl. Disease and Trait Mapping Genetics)
10 Technology > 1001 Agricultural Biotechnology > 100105 Genetically Modified Field Crops and Pasture
07 Agricultural and Veterinary Sciences > 0703 Crop and Pasture Production > 070305 Crop and Pasture Improvement (Selection and Breeding)
Socio-Economic Objective (SEO2008): B Economic Development > 82 Plant Production and Plant Primary Products > 8205 Winter Grains and Oilseeds > 820507 Wheat
Identification Number or DOI: doi: 10.1071/AR05248
URI: http://eprints.usq.edu.au/id/eprint/2035

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