Changes in the composition of the extracellular matrix in patellar tendinopathy

Samiric, Tom and Parkinson, John and Ilic, Mirna Z. and Cook, Jill and Feller, Julian A. and Handley, Christopher J. (2009) Changes in the composition of the extracellular matrix in patellar tendinopathy. Matrix Biology, 28 (4). pp. 230-236. ISSN 0945-053X

Abstract

Objective: To compare the chemical levels and mRNA expression of proteoglycan and collagen in normal human patellar tendons and tendons exhibiting chronic overuse tendinopathy. Methods: Sulfated glycosaminoglycan and hydroxyproline content were investigated by spectrophotometric measurement using papain-digested samples. Deglycosylated proteoglycan core proteins were analysed by Western blot using specific antibodies. Total mRNA isolated from samples of frozen tendons was assayed by relative quantitative RT-PCR for decorin, biglycan, fibromodulin, versican, aggrecan, and collagens Type I, II and III and normalised to glyceraldehyde-3-phosphate dehydrogenase. Results: There was a significant increase in sulfated glycosaminoglycan content in pathologic tendons compared to normal. This was attributed to an increased deposition of the large aggregating proteoglycans versican and aggrecan and the small proteoglycans biglycan and fibromodulin, but not decorin. Aggrecan and versican were extensively degraded in both normal and pathologic tendons, biglycan was more fragmented in the pathologic tendons while predominantly intact fibromodulin and decorin were present in normal and pathologic tendons. There was a greater range in total collagen content but no change in the level of total collagen in pathologic tendons. There were no significant differences between the pathologic and normal tendon for all genes, however p values close to 0.05 indicated a trend in downregulation of Type I collagen and fibromodulin, and upregulation in versican and Type III genes in pathologic tissue. Conclusion: The changes in proteoglycan and collagen levels observed in patellar tendinopathy appear to be primarily due to changes in the metabolic turnover of these macromolecules. Changes in the expression of these macromolecules may not play a major role in this process.


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Item Type: Article (Commonwealth Reporting Category C)
Refereed: Yes
Item Status: Live Archive
Additional Information: Permanent restricted access to published version due to publisher copyright policy.
Depositing User: Dr John Parkinson
Faculty / Department / School: Historic - Faculty of Sciences - Department of Biological and Physical Sciences
Date Deposited: 23 Sep 2011 03:52
Last Modified: 03 Jul 2013 00:42
Uncontrolled Keywords: collagen; patellar tendinopathy; proteoglycans; tendon
Fields of Research (FOR2008): 11 Medical and Health Sciences > 1106 Human Movement and Sports Science > 110601 Biomechanics
11 Medical and Health Sciences > 1103 Clinical Sciences > 110314 Orthopaedics
10 Technology > 1004 Medical Biotechnology > 100403 Medical Molecular Engineering of Nucleic Acids and Proteins
Socio-Economic Objective (SEO2008): C Society > 92 Health > 9201 Clinical Health (Organs, Diseases and Abnormal Conditions) > 920116 Skeletal System and Disorders (incl. Arthritis)
Identification Number or DOI: doi: 10.1016/j.matbio.2009.04.001
URI: http://eprints.usq.edu.au/id/eprint/19330

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