Salicylic acid increased aldose reductase activity and sorbitol accumulation in tomato plants under salt stress

Tari, I. and Kiss, G. and Deer, Aranka K. and Csiszar, J. and Erdei, L. and Galle, A. and Gemes, Katalin and Horvath, Ferenc and Poor, Peter and Szepesi, Agota and Simon, L. M. (2010) Salicylic acid increased aldose reductase activity and sorbitol accumulation in tomato plants under salt stress. Biologia Plantarum: Journal for Experimental Botany, 54 (4). pp. 677-683. ISSN 0006-3134

Abstract

Increased aldose reductase (ALR) activities were detected in the leaf tissues of tomato plants grown for 3 weeks in culture medium containing 10-7 or 10-4 M salicylic acid (SA), and in the roots after the 10-4 M SA pretreatment. The ALR activity changed in parallel with the sorbitol content in the leaves of the SA-treated plants. Salt stress elicited by 100 mM NaCl enhanced the accumulation of sorbitol in the leaves of control plants and as compared with the untreated control the sorbitol content in the SA-pretreated leaves remained elevated under salt stress. DEAE cellulose anionexchange column purification of the protein precipitated with 80 % (NH4)2SO4 revealed two enzyme fractions with ALR activity in both the leaf and the root tissues. The fraction of the leaf extract that was not bound to the column reacted with glucose and glucose-6-P as substrates, whereas glucose was not a substrate for the bound fraction or for root isoenzymes. The root enzyme was less sensitive to salt treatment: 50 mM NaCl caused 30 % inhibition in the leaf extract, whereas the enzyme activity of the root extract was not affected. It is suggested that increased ALR activity and sorbitol synthesis in the leaves of SA-treated tomato plants may result in an improved salt stress tolerance.


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Item Type: Article (Commonwealth Reporting Category C)
Refereed: Yes
Item Status: Live Archive
Additional Information: Permanent restrcited access to published version due to publisher copyright restrictions.
Depositing User: epEditor USQ
Faculty / Department / School: Historic - Faculty of Engineering and Surveying - Department of Agricultural, Civil and Environmental Engineering
Date Deposited: 31 May 2011 11:47
Last Modified: 20 Oct 2014 04:13
Uncontrolled Keywords: ALR activity in vitro; enzyme purification; lycopersicon esculentum; NaCl
Fields of Research (FOR2008): 06 Biological Sciences > 0607 Plant Biology > 060702 Plant Cell and Molecular Biology
07 Agricultural and Veterinary Sciences > 0703 Crop and Pasture Production > 070303 Crop and Pasture Biochemistry and Physiology
10 Technology > 1001 Agricultural Biotechnology > 100101 Agricultural Biotechnology Diagnostics (incl. Biosensors)
Socio-Economic Objective (SEO2008): B Economic Development > 82 Plant Production and Plant Primary Products > 8202 Horticultural Crops > 820299 Horticultural Crops not elsewhere classified
Identification Number or DOI: doi: 10.1007/s10535-010-0120-1
URI: http://eprints.usq.edu.au/id/eprint/19132

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