Dearnaley, John and Learmonth, Barbara A. and Bougoure, Damian S. (2002) Phosphate transporter expression in the soybean AM symbiosis. In: 7th International Mycological Congress (IMC7), 11-17 Aug 2002, Oslo, Norway.
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We are currently studying genes that are potentially involved in the process of phosphate uptake in the arbuscular mycorrhizal (AM) symbiosis of soybean. We have extracted mRNA from AM colonised and uncolonised soybean roots using biotinylated poly-T probes and streptavidin-coated magnetic particles. Total mRNA extracted by this method was reverse-transcribed into cDNA with poly-T primer and reverse transcriptase. cDNA was next PCR-amplified with primers for a previously published soybean root phosphate transporter gene and amplified sequences were visualized on silver-stained acrylamide gels. In situ hybridization studies were carried out to localise the phosphate transporter in root tissues. Root cryosections were probed with a fluorosceinconjugated antisense probe and bound targets were visualized either via alkaline phosphatase labeling or with an antifluoroscein texas-red antibody. Results of these investigations are presented here.
|Item Type:||Conference or Workshop Item (Commonwealth Reporting Category E) (Paper)|
|Additional Information:||Full paper not available.|
|Uncontrolled Keywords:||soybean; phosphate uptake; mycorrhizal symbiosis|
|Fields of Research (FOR2008):||06 Biological Sciences > 0604 Genetics > 060499 Genetics not elsewhere classified|
|Socio-Economic Objective (SEO2008):||E Expanding Knowledge > 97 Expanding Knowledge > 970106 Expanding Knowledge in the Biological Sciences|
|Deposited On:||23 Mar 2011 10:56|
|Last Modified:||23 Mar 2011 12:30|
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