Effect of L-arginine on cardiac function and fibrosis in mdx mice

Hoey, Andrew and van Erp, Christel (2006) Effect of L-arginine on cardiac function and fibrosis in mdx mice. In: Combined ASBMB, ASPS, AuPS, ANZSCDB, NZSBMB, NZSPP, 24-28 Sep 2006, Brisbane, Australia.

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Abstract

The primary cause of Duchenne Muscular Dystrophy, a condition that affects 1 in 3500 live male births, is a deficiency of the protein dystrophin. This deficiency leads to downregulation of the neuronal nitric oxide synthase (nNOS) and lowered production of nitric oxide. This study investigated the effects of L-arginine, the substrate of nNOS, on cardiac function and fibrosis in mdx mice, a widely used animal model for Duchenne Muscular Dystrophy. Six month old mice received 5 mg/g body weight L-arginine by daily oral gavage for six months. At the completion of treatment, mice were anaesthetised with sodium pentobarbitone (70 mg/kg, ip) prior to euthanasia by excision of the heart. Cardiac function was assessed with the Langendorff technique at a perfusion pressure of 80 mmHg. Mdx mice had an impaired left ventricular developed pressure relative to C57BL10ScSn control mice, but this difference was not evident in L-arginine treated mdx mice. The L-arginine treated mdx mice also showed an increased coronary flow and reduced diastolic stiffness relative to untreated mdx mice (P<0.05). This reduction in stiffness was associated with a significant reduction in cardiac collagen measured as the percent of left ventricular area stained with the collagen specific dye picrosirius red (P<0.05). These results indicate that chronic treatment with L-arginine produces the beneficial effects of improving function and reducing fibrosis in the dystrophin-deficient heart.


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Item Type: Conference or Workshop Item (Commonwealth Reporting Category E) (Poster)
Refereed: No
Item Status: Live Archive
Additional Information: Poster presentation. Abstract only available. No evidence of copyright restrictions on web site.
Depositing User: Ms Debbie White
Faculty / Department / School: Historic - Faculty of Sciences - Department of Biological and Physical Sciences
Date Deposited: 11 Oct 2007 00:44
Last Modified: 25 Sep 2013 02:21
Uncontrolled Keywords: Duchenne Muscular Dystrophy, L-arginine, cardiac function, fibrosis, mdx mice
Fields of Research (FOR2008): 06 Biological Sciences > 0606 Physiology > 060699 Physiology not elsewhere classified
URI: http://eprints.usq.edu.au/id/eprint/1472

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