Use of fluorescence spectroscopy to differentiate yeast and bacterial cells

Bhatta, H. and Goldys, Ewa M. and Learmonth, Robert P. (2006) Use of fluorescence spectroscopy to differentiate yeast and bacterial cells. Applied Microbiology and Biotechnology, 71 (1). pp. 121-126. ISSN 0175-7598

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Abstract

This study focuses on the characterization of bacterial and yeast species through their autofluorescence spectra. Lactic acid bacteria (Lactobacillus sp.), and yeast(Saccharomyces sp.) were cultured under controlled conditions and studied for variations in their autofluorescence, particularly in the area representative of tryptophan residues of proteins. The emission and excitation spectra clearly reveal that bacterial and yeast species can be differentiated by their intrinsic fluorescence with UV excitation. The possibility of differentiation between different strains of Saccharomyces yeast was also studied, with clear differences observed for selected strains. The study shows that fluorescence can be successfully used to differentiate between yeast and bacteria and between different yeast species, through the identification of spectroscopic fingerprints, without the need for fluorescent staining.


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Item Type: Article (Commonwealth Reporting Category C)
Refereed: Yes
Item Status: Live Archive
Additional Information: The original publication is available at: http://www.springerlink.com/content/b0146k3r2j25716n/fulltext.pdf
Depositing User: Assoc Prof Robert Learmonth
Faculty / Department / School: Historic - Faculty of Sciences - Department of Biological and Physical Sciences
Date Deposited: 11 Oct 2007 00:34
Last Modified: 02 Jul 2013 22:35
Uncontrolled Keywords: fluorescence spectroscopy; bacteria; yeast; differentiation
Fields of Research (FOR2008): 03 Chemical Sciences > 0306 Physical Chemistry (incl. Structural) > 030606 Structural Chemistry and Spectroscopy
10 Technology > 1003 Industrial Biotechnology > 100305 Industrial Microbiology (incl. Biofeedstocks)
10 Technology > 1003 Industrial Biotechnology > 100304 Industrial Biotechnology Diagnostics (incl. Biosensors)
Socio-Economic Objective (SEO2008): E Expanding Knowledge > 97 Expanding Knowledge > 970106 Expanding Knowledge in the Biological Sciences
Identification Number or DOI: doi: 10.1007/s00253-005-0309-y
URI: http://eprints.usq.edu.au/id/eprint/1062

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